PXD064722 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative proteomics of U2O2 cells in asynchronous or synchronized in late mitosis |
| Description | U2OS cells were either asynchronous or synchronized in late mitosis (anaphase/telophase) prior to irradiation. Asynchronous cells were grown to 75–80% confluence, irradiated with 4 Gy, and collected 5–10 minutes later. For synchronized samples, cells were subjected to a thymidine block, released into S phase, and then arrested in prometaphase with nocodazole. Mitotic cells were collected by shake-off, washed, and incubated in fresh medium for 35–40 minutes to allow progression into anaphase/telophase before irradiation with 4 Gy. Cell pellets were snap-frozen in liquid nitrogen for proteomics. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-18 |
| AnnouncementXML | Submission_2026-03-18_08:30:26.727.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Inmaculada Jorge |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-06 05:05:10 | ID requested | |
| ⏵ 1 | 2026-03-18 08:30:27 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: U2O2 cell, irradiation, mitosis,quantitative proteomics |
Contact List
| Jesús Vázquez Cobos |
|---|
| contact affiliation | Spanishg National Center for Cardiovascular Research (CNIC), Madrid, Spain. |
| contact email | jesus.vazquez@cnic.es |
| lab head | |
| Inmaculada Jorge |
|---|
| contact affiliation | Cardiovascular Proteomics Lad. Spanish National Center for Cardiovascular Research (CNIC). Madrid. |
| contact email | ijorge@cnic.es |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064722 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064722
- Label: PRIDE project
- Name: Quantitative proteomics of U2O2 cells in asynchronous or synchronized in late mitosis
