PXD064689 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mtr complex and MtrI quantification from Methanosarcina mazei |
| Description | This dataset contains LC-MS/MS data from multiple experiments investigating the N⁵-methyltetrahydromethanopterin:CoM-S-methyltransferase (Mtr) complex of Methanosarcina mazei, with quantitative analysis performed using MaxQuant. Experiment 1: Strep-Tactin-based purification of the native Mtr complex from M. mazei grown on methanol. Samples were divided into two conditions: Group 1: Purification performed under aerobic conditions Group 2: Purification performed under anaerobic conditions Each condition was analyzed in biological triplicates. The aim was to quantify the relative abundance of MtrI (Uniprot ID: Q8PUD4) compared to other Mtr subunits, particularly MtrA. Experiment 2: Similar to Experiment 1, but cells were grown on H₂/CO₂ instead of methanol. Group 1: Aerobic purification Group 2: Anaerobic purification Again, biological triplicates were performed for each condition. The objective remained the same: to quantify MtrI in both groups relative to other Mtr subunits. Experiment 3: Reciprocal pulldown of Mtr subunits using His-tagged MtrI expressed in M. mazei, purified via Ni-NTA affinity chromatography. Empty vector control purifications were included to assess specificity of interactions. Full experimental details are available in the associated manuscript (Reif-Trauttmansdorff et al., 2025, in preparation). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-19 |
| AnnouncementXML | Submission_2026-01-18_16:17:51.495.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tristan Reif-Trauttmansdorff |
| SpeciesList | scientific name: Methanosarcina mazei Go1; NCBI TaxID: NEWT:192952; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-05 08:11:57 | ID requested | |
| ⏵ 1 | 2026-01-18 16:17:52 | announced | |
Publication List
| 10.1038/s41467-025-67705-5; |
| Reif-Trauttmansdorff T, Herdering E, Bohn S, Pascoa T, Kahnt J, Zimmer E, Kumar A, Schmitz RA, Schuller JM, Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI. Nat Commun, 17(1):133(2025) [pubmed] |
Keyword List
| submitter keyword: Archaea,Methanogen, Methanosarcina, Mtr complex, MaxQuant, small protein |
Contact List
| Jan Schuller |
|---|
| contact affiliation | Center for Synthetic Microbiology (SYNMIKRO) Research Center and Department of Chemistry, Philipps-Universität Marburg, Karl-von-Frisch Straße 14, 35043 Marburg |
| contact email | jan.schuller@synmikro.uni-marburg.de |
| lab head | |
| Tristan Reif-Trauttmansdorff |
|---|
| contact affiliation | University of Marburg |
| contact email | reiftrau@staff.uni-marburg.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064689
- Label: PRIDE project
- Name: Mtr complex and MtrI quantification from Methanosarcina mazei
