PXD064689

PXD064689 is an original dataset announced via ProteomeXchange.

Title	Mtr complex and MtrI quantification from Methanosarcina mazei
Description	This dataset contains LC-MS/MS data from multiple experiments investigating the N⁵-methyltetrahydromethanopterin:CoM-S-methyltransferase (Mtr) complex of Methanosarcina mazei, with quantitative analysis performed using MaxQuant. Experiment 1: Strep-Tactin-based purification of the native Mtr complex from M. mazei grown on methanol. Samples were divided into two conditions: Group 1: Purification performed under aerobic conditions Group 2: Purification performed under anaerobic conditions Each condition was analyzed in biological triplicates. The aim was to quantify the relative abundance of MtrI (Uniprot ID: Q8PUD4) compared to other Mtr subunits, particularly MtrA. Experiment 2: Similar to Experiment 1, but cells were grown on H₂/CO₂ instead of methanol. Group 1: Aerobic purification Group 2: Anaerobic purification Again, biological triplicates were performed for each condition. The objective remained the same: to quantify MtrI in both groups relative to other Mtr subunits. Experiment 3: Reciprocal pulldown of Mtr subunits using His-tagged MtrI expressed in M. mazei, purified via Ni-NTA affinity chromatography. Empty vector control purifications were included to assess specificity of interactions. Full experimental details are available in the associated manuscript (Reif-Trauttmansdorff et al., 2025, in preparation).
HostingRepository	PRIDE
AnnounceDate	2026-01-19
AnnouncementXML	Submission_2026-01-18_16:17:51.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tristan Reif-Trauttmansdorff
SpeciesList	scientific name: Methanosarcina mazei Go1; NCBI TaxID: NEWT:192952;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-05 08:11:57	ID requested
⏵ 1	2026-01-18 16:17:52	announced

10.1038/s41467-025-67705-5;

Reif-Trauttmansdorff T, Herdering E, Bohn S, Pascoa T, Kahnt J, Zimmer E, Kumar A, Schmitz RA, Schuller JM, Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI. Nat Commun, 17(1):133(2025) [pubmed]

submitter keyword: Archaea,Methanogen, Methanosarcina, Mtr complex, MaxQuant, small protein

Jan Schuller
contact affiliation	Center for Synthetic Microbiology (SYNMIKRO) Research Center and Department of Chemistry, Philipps-Universität Marburg, Karl-von-Frisch Straße 14, 35043 Marburg
contact email	jan.schuller@synmikro.uni-marburg.de
lab head
Tristan Reif-Trauttmansdorff
contact affiliation	University of Marburg
contact email	reiftrau@staff.uni-marburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064689
     1. Label: PRIDE project
     2. Name: Mtr complex and MtrI quantification from Methanosarcina mazei