PXD064677-1

PXD064677 is an original dataset announced via ProteomeXchange.

Title	Control of flagellar gene expression by the receptor-like regulator in pathogenic Escherichia coli
Description	Chemoreceptors enable bacteria to modulate their swimming behavior in accordance with the perceived environmental cues. Bacteria exhibit large diversity of stimuli sensed by their chemoreceptors, whereas the output of receptors to the chemotaxis signaling pathway that controls flagellar motor is typically highly conserved. Here, we characterize a unique chemoreceptor-like protein, Tls, which is found in the B2 phylogroup of Escherichia coli that includes many extraintestinal pathogenic (ExPEC) strains. Instead of mediating chemotactic signaling, Tls controls motility by repressing the expression of flagellar genes, and thus cell motility, apparently by sequestering the transcriptional master activator of flagellar genes, FlhDC. Furthermore, we observe that the subcellular localization of Tls, the sequestration of FlhDC, and the repression of flagellar genes and motility are all abolished during growth on porous medium, indicating that this regulation may be mechanosensitive. Deletion of tls in a uropathogenic E. coli leads to reduced attachment to the urinary tract cells, but an increased migration to and/or proliferation in the murine gut, a pathogen reservoir niche, thus implicating Tls in the regulation of motility during infection.
HostingRepository	PRIDE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-12_23:36:41.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-05 05:12:38	ID requested
⏵ 1	2025-06-12 23:36:42	announced

Dataset with its publication pending

submitter keyword: E.coli

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064677
     1. Label: PRIDE project
     2. Name: Control of flagellar gene expression by the receptor-like regulator in pathogenic Escherichia coli