PXD064621-1

PXD064621 is an original dataset announced via ProteomeXchange.

Title	miR-940 suppresses ferroptosis by limiting hallmarks of ferroptotic cell death
Description	Ferroptosis is a form of regulated cell death that is characterized by iron-dependent lipid peroxidation. This process is regulated by specific metabolites, the lipid composition of the cells, and antioxidant mechanisms. Although many regulators of ferroptosis have been identified over the past decade, further exploration of other cellular mechanisms, particularly those originating from non-coding regions, is necessary to develop a thorough understanding of the multifaceted regulatory processes underlying ferroptosis. MicroRNAs (miRNAs) play a crucial role in gene regulation and cellular functions. Through a CRISPR-KO screen, we identified miR-940 as a negative regulator of ferroptosis. Bioinformatics analysis, RNAseq, and proteomics revealed that miR-940 suppresses the expression of ACSL4, LPCAT3, DMT1, and NCOA4. In parallel, it promotes the expression of the ferroptosis gatekeeper GPX4. This gene rewiring reduces levels of redox-active iron and diminishes lipid peroxidation, thereby suppressing ferroptosis. Together, these findings suggest a central role for miR-940 in coordinating the inhibition of ferroptosis, presenting a novel regulatory layer for therapeutic exploration.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:25:46.248.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliane Merl-Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-04 05:14:11	ID requested
⏵ 1	2026-09-06 16:25:47	announced

10.1002/advs.75830;

Kolak A, Tschuck J, Wei, ß SAI, Kaemena D, Klimm K, Galhoz A, Ringelstetter L, Fennell M, Merl-Pham J, Artati A, Strasser S, Garippa R, Witting M, Zischka H, Schick JA, Hauck SM, Menden MP, Vincendeau M, Stockwell BR, Hadian K, MiR-940 Suppresses Ferroptosis by Controlling Expression of Key Regulatory Genes. Adv Sci (Weinh), 13(46):e75830(2026) [pubmed]

submitter keyword: microRNA

miR-940

ferroptosis

lipid peroxidation

Kamyar Hadian
contact affiliation	Cell Signaling and Chemical Biology, Research Unit Signaling and Translation, Helmholtz Zentrum München, Neuherberg, Germany; Department of Biological Sciences, Department of Chemistry, Herbert Irving Comprehensive Cancer Center, Irving Institute for Cancer Dynamics, Columbia University, New York, NY, USA.
contact email	kamyar.hadian@helmholtz-munich.de
lab head
Juliane Merl-Pham
contact affiliation	Metabolomics and Proteomics Core, Helmholtz Munich
contact email	juliane.merl@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD064621

PRIDE project URI

• PRIDE
  1. PXD064621
     1. Label: PRIDE project
     2. Name: miR-940 suppresses ferroptosis by limiting hallmarks of ferroptotic cell death