PXD064618-1

PXD064618 is an original dataset announced via ProteomeXchange.

Title	A clinic responder-derived defined microbial consortium enhances anti-PD-1 immunotherapy efficacy in mice
Description	Growing evidence supports the gut microbiota as a promising target for enhancing the efficacy of cancer immunotherapy. However, the success of microbiota-targeting interventions has been limited, partly due to interindividual microbial heterogeneity. Here, we designed and constructed a defined consortium (RCom) of 15 bacterial species that are associated with improved clinic response to anti-PD-1 treatment and were mostly isolated from the feces of responder patients. Through in silico prediction and experimental characterization in vitro and in vivo, we demonstrated that the RCom is a relatively cooperative community, in which five Bacteroidetes species and ten Firmicutes species act together to establish optimal engraftment and produce immunomodulatory metabolites. Despite a profound heterogeneity of the baseline gut microbiota, oral supplementation of the RCom consistently improved the anti-tumor activity of anti-PD-1 by increasing intratumoral infiltration and cytotoxic activity of CD8+ T cells in syngeneic tumor models. Moreover, administration of the RCom circumvented the anti-PD-1 resistance in mice conferred by fecal microbiota transplantation from individual non-responsive patients. The RCom is thus a potential adjuvant to augment responsiveness to anti-PD-1 therapy in cancer
HostingRepository	PRIDE
AnnounceDate	2026-01-14
AnnouncementXML	Submission_2026-01-14_00:10:38.521.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haiyan Zhou
SpeciesList	scientific name: Firmicutes bacterium HGW-Firmicutes-5; NCBI TaxID: NEWT:2013786;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-04 04:15:55	ID requested
⏵ 1	2026-01-14 00:10:39	announced

Dataset with its publication pending

submitter keyword: None

Chen Yang
contact affiliation	State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China
contact email	yang.c@sjtu.edu.cn
lab head
Haiyan Zhou
contact affiliation	Shanghai Jiao Tong University
contact email	haiyan5555@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064618
     1. Label: PRIDE project
     2. Name: A clinic responder-derived defined microbial consortium enhances anti-PD-1 immunotherapy efficacy in mice