PXD064601 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Shotgun Proteomic Identification of Amphotericin B Targets in Late Endosomes of Influenza A Virus-Infected A549 Cells |
| Description | Amphotericin B (AmB), a polyene macrolide antifungal, is well known for its ability to bind ergosterol and cholesterol; however, its interactions with host proteins remain poorly characterized. In this study, we aimed to identify AmB-binding targets in A549 cells infected with the influenza A virus (H1N1) strain A/PR/8/34 (PR8). We isolated late endosomal fractions and performed a biotin–streptavidin pulldown using a biotinylated AmB probe, with specificity confirmed by competition with excess free AmB. The proteins enriched from these fractions were then analyzed by shotgun LC–MS/MS, revealing a set of late endosomal proteins that specifically interact with AmB during influenza A virus infection. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-02 |
| AnnouncementXML | Submission_2026-02-02_07:07:24.027.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Di He |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-03 23:51:17 | ID requested | |
| ⏵ 1 | 2026-02-02 07:07:24 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Amphotericin B |
| Influenza A virus |
| Late endosome |
| Shotgun proteomics |
Contact List
| Di He |
|---|
| contact affiliation | China-Japan Friendship Hospital, Beijing, China |
| contact email | hd980122@163.com |
| lab head | |
| Di He |
|---|
| contact affiliation | China-Japan Friendship Hospital |
| contact email | hd980122@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064601 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064601
- Label: PRIDE project
- Name: Shotgun Proteomic Identification of Amphotericin B Targets in Late Endosomes of Influenza A Virus-Infected A549 Cells
