PXD064577-1

PXD064577 is an original dataset announced via ProteomeXchange.

Title	Dynamic sperm proteome remodelling during epididymal maturation in a marsupial, Macropus giganteus
Description	While assisted reproductive technologies (ARTs) are widely used in domestic animals, successful implementation of ARTs to conserve wildlife species remains challenging. In macropods, crucial aspects of fundamental reproductive biology, including changes induced by epididymal maturation, remain unknown, limiting the development of ARTs. In this context we performed a proteomic analysis of spermatozoa from the caput, corpus, and cauda epididymis of Eastern Grey Kangaroos (n = 6) to profile changes over epididymal maturation. Samples prepared by FASP digestion were analysed by LC-MS/MS with SWATH acquisition. A total of 4,304 proteins were identified, with significant overlap across epididymal regions. Highly abundant proteins in common across caput, corpus and cauda spermatozoa had strong enrichment for tubulins and included 4 histone proteins. The most significant proteomic remodelling was observed in the corpus to cauda transition, late in epididymal transit (728 differentially abundant proteins). Overall proteomic changes across epididymal maturation (1,131 differentially abundant proteins) suggested a loss of sperm glycosidases and an increase in flagellar proteins, including tubulins and dyneins. These findings serve to highlight both consistencies with eutherian sperm epididymal maturation (e.g. bias towards protein loss over transit, transfer of proteins via extracellular vesicles) and elements which are likely unique to marsupials (e.g. reduced chromatin stability, potential use of β-oxidation as a major metabolic pathway). This critical information can now be leveraged to further develop ARTs in marsupials.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:16:10.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taylor Pini
SpeciesList	scientific name: Macropus giganteus; NCBI TaxID: 9317;
ModificationList	No PTMs are included in the dataset
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 15:26:51	ID requested
⏵ 1	2025-09-28 16:16:11	announced

10.1093/biolre/ioaf160;

Kunkel S, Johnston SD, Nouwens A, Pini T, . Biol Reprod, 113(3):581-591(2025) [pubmed]

submitter keyword: epididymal, sperm,kangaroo, marsupial, macropod, epididymis, spermatozoa

Taylor Pini
contact affiliation	University of Queensland, School of Veterinary Science, Faculty of Science
contact email	t.pini@uq.edu.au
lab head
Taylor Pini
contact affiliation	University of Queensland
contact email	t.pini@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064577

PRIDE project URI

• PRIDE
  1. PXD064577
     1. Label: PRIDE project
     2. Name: Dynamic sperm proteome remodelling during epididymal maturation in a marsupial, Macropus giganteus