PXD064570-1

PXD064570 is an original dataset announced via ProteomeXchange.

Title	Large scale cerebrospinal fluid proteomics reveals biomarkers of Multiple Sclerosis
Description	Cerebrospinal fluid (CSF) plays critical roles in clinical diagnostics, offers a window into neuropathological mechanisms and is available at scale. To enable its large-scale proteomics characterization, we developed a high-throughput mass spectrometry-based workflow based on the Orbitrap Astral. This workflow quantifies about 2100 proteins per sample at a throughput of 100 samples per day. We measured 2600 CSF samples across a discovery and replication cohort from patients with multiple sclerosis and related inflammatory CNS conditions as well as neurological controls. Analysis of CSF proteomes yielded a panel of 29 protein biomarkers to identify MS with better diagnostic performance than established clinical CSF markers (QIgG, QAlb, leukocyte count) amongst patients presenting with MS-like symptoms but without CSF-specific oligoclonal bands.
HostingRepository	PRIDE
AnnounceDate	2025-12-14
AnnouncementXML	Submission_2025-12-13_18:15:58.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 09:39:55	ID requested
⏵ 1	2025-12-13 18:15:58	announced

Dataset with its publication pending

submitter keyword: Multiple Sclerosis, body fluids, Astral,CSF, biomarkers

Matthias Mann
contact affiliation	Department of Proteomics and Signal Transduction, Max Planck Institute of Biochmistry, Martinsried, Munich, Germany
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064570
     1. Label: PRIDE project
     2. Name: Large scale cerebrospinal fluid proteomics reveals biomarkers of Multiple Sclerosis