PXD064558-1

PXD064558 is an original dataset announced via ProteomeXchange.

Title	Analysis of the Impact of Exposure to Gut Microbiota Dysbiosis on Early Life Cecal Acetylome
Description	The period from birth to two years is the phase of the fastest growth and development in children, as well as an important window for the development of intestinal microbiota. Dysbiosis of the gut microbiome can lead to various adverse conditions in children, including malabsorption and immune abnormalities, ultimately resulting in a series of negative events related to growth and development. Lysine acetylation, as a significant post-translational modification, plays a complex and crucial role in the regulation of gut microbiota. This study aims to investigate the mechanism by which ABX-induced lysine acetylation affects the abnormal physiological state simulating gut microbiota dysbiosis in children. In this study, we identified a total of 16,579 acetylation sites from 5,218 proteins. We found that antibiotic-induced dysbiosis in young mice (3 weeks) can cause extensive changes in the lysine acetylation and proteomic profiles of cecal tissue. Differentially acetylated proteins are involved in various metabolic pathways, including the citrate cycle (TCA) cycle, butanoate metabolism, pyruvate metabolism, glycolysis/gluconeogenesis, and fatty acid biosynthesis. These differential acetylation sites are distributed across the cytoplasm, nucleus, and mitochondria, suggesting that multiple cellular functions are involved in regulation. Our findings suggest that early-life gut microbiota dysbiosis may lead to a series of metabolic disorders by regulating lysine acetylation in cecal tissue, resulting in delayed growth and development. This study aims to provide valuable insights into the molecular mechanisms underlying a series of pathophysiological processes caused by early-life gut microbiota dysbiosis. It contributes to a deeper understanding of the consequences of acetylation changes associated with early-life gut microbiota dysbiosis and its potential role in metabolic disorders.
HostingRepository	PRIDE
AnnounceDate	2025-09-15
AnnouncementXML	Submission_2025-09-14_16:35:32.437.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yubing Zeng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 03:08:38	ID requested
⏵ 1	2025-09-14 16:35:33	announced

10.3390/biology14080917;

Zeng Y, Shen J, He X, Liu F, Wang Y, Wang Y, Qiao Y, Pei P, Wang S, Gut Microbiota Dysbiosis Remodels the Lysine Acetylome of the Mouse Cecum in Early Life. Biology (Basel), 14(8):(2025) [pubmed]

submitter keyword: gut microbiota dysbiosis

early life

acetylome

Shan Wang, Yubing Zeng
contact affiliation	1、Capital Institute of Pediatrics,Chinese Academy of Medical Sciences & Peking Union Medical College 2、Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics
contact email	wsaquarius@sina.com
lab head
Yubing Zeng
contact affiliation	Capital Institute of Pediatrics
contact email	awater007@sina.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064558
     1. Label: PRIDE project
     2. Name: Analysis of the Impact of Exposure to Gut Microbiota Dysbiosis on Early Life Cecal Acetylome