PXD064542 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Small Molecule Inhibition of VDAC1 Reroutes Mitochondrial Metabolite Flux |
| Description | Mitochondria support cancer cell proliferation by producing ATP and supplying metabolic intermediates needed for macromolecule synthesis. Voltage-Dependent Anion Channels (VDACs), particularly isoform 1 (VDAC1), regulate mitochondrial metabolite flux and serve as metabolic gatekeepers. We recently identified SC18, a small-molecule inhibitor targeting the NADH-binding pocket of VDAC1, which reduces mitochondrial membrane potential, NADH, and ATP levels, and synergizes with FDA-approved hepatocarcinoma drugs. In this study, we used structural biology, electrophysiology, proteomics, and metabolic assays to show that SC18 binds cooperatively within the NADH-binding pocket, enhancing VDAC closure in the presence of NADH. This impairs mitochondrial uptake of key substrates and initiates compensatory metabolic changes, ultimately disrupting mitochondrial bioenergetics and cancer cell viability. Our findings provide a mechanistic basis for targeting VDAC1 to disrupt cancer metabolism. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_02:47:21.920.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Akos Vegvari |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-03 00:26:28 | ID requested | |
| ⏵ 1 | 2026-08-25 02:47:22 | announced | |
| 2 | 2026-08-25 02:50:51 | announced | 2026-08-25: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: drug,VDAC, mitochondria, liver cancer cells, cancer metabolism |
Contact List
| Akos Vegvari |
|---|
| contact affiliation | Karolinska Institutet, Stockholm, Sweden |
| contact email | akos.vegvari@ki.se |
| lab head | |
| Akos Vegvari |
|---|
| contact affiliation | Karolinska Institutet |
| contact email | akos.vegvari@ki.se |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD064542 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064542
- Label: PRIDE project
- Name: Small Molecule Inhibition of VDAC1 Reroutes Mitochondrial Metabolite Flux
