PXD064533-1

PXD064533 is an original dataset announced via ProteomeXchange.

Title	Mouse Plasma Extracellular Vesicle Proteome
Description	Regular physical activity elicits broad physiological adaptations, yet the molecular mechanisms underlying systemic responses remain incompletely understood. Circulating extracellular vesicles (EVs) have emerged as key mediators of interorgan communication, carrying protein cargo that may reflect and influence tissue adaptation to exercise. This study aimed to characterize the proteomic profile of plasma-derived EVs (ExerVs) from exercised versus sedentary mice to uncover candidate proteins involved in exercise-induced remodeling and metabolic regulation. EVs were isolated from plasma of mice subjected to four weeks of voluntary wheel running, followed by untargeted LC-MS/MS analysis using data-dependent acquisition-PASEF on a timsTOF Pro 2. Label-free quantification was performed to identify differentially expressed proteins. These findings provide insight into the EV-mediated mechanisms of exercise adaptation and support future translational efforts to identify systemic exercise-responsive biomarkers and therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:39:41.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanqi Tan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-02 18:36:05	ID requested
⏵ 1	2026-06-28 16:39:42	announced

10.1096/fj.202505096rr;

Fliflet AM, Spradlin RA, Tan Y, Nishitha Vijayan A, Choi SJ, Kao WC, Aksamitiene E, Nelappana M, Ding S, Huang KY, Joshi A, Kambar N, Bludgen T, Meehan M, Boss JL, Knipp M, Fan H, Leal C, Sunde RA, Dobrucki LW, Boppart SA, Kong HJ, Sweedler JV, Boppart MD, Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)-Enriched Extracellular Vesicles That Promote Angiogenesis. FASEB J, 40(12):e72052(2026) [pubmed]

submitter keyword: Exercise,Mouse, Extracellular Vesicles, Plasma, Proteomics, LC-MS/MS

jonathan sweedler
contact affiliation	University of Illinois Urbana-Champaign
contact email	jsweedle@illinois.edu
lab head
Yanqi Tan
contact affiliation	University of Illinois Urbana Champaign
contact email	tan61@illinois.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064533

PRIDE project URI

• PRIDE
  1. PXD064533
     1. Label: PRIDE project
     2. Name: Mouse Plasma Extracellular Vesicle Proteome