PXD064530 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cleavage preference of metalloprotease EA1 |
| Description | The cleavage preference of the thermophilic metalloprotease from Geobacillus sp. EA1 was characterised using mass spectrometry, confirming its similarity to thermolysin in preferring P1’ hydrophobic amino acids. While EA1 showed similar efficiencies for cleavage of short peptides with one of six hydrophobic amino acids at the P1’ position, thermolysin showed marked preference for leucine. A single amino acid difference in the S1’ pocket of these similar enzymes provides a rationale for this difference. A variant of EA1 (F133L) had intermediary preference, suggesting that approximately half the difference in preference is attributable to this single amino acid change. Broader preference and higher efficiency make EA1 a superior candidate for quick digestion of varied protein substrates. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-25 |
| AnnouncementXML | Submission_2025-11-25_11:42:39.596.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Torsten Kleffmann |
| SpeciesList | scientific name: Bacillus thermoproteolyticus; NCBI TaxID: NEWT:1427; scientific name: Escherichia coli; NCBI TaxID: NEWT:562; scientific name: Geobacillus sp. EFT1; NCBI TaxID: NEWT:1108144; |
| ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-02 15:30:16 | ID requested | |
| ⏵ 1 | 2025-11-25 11:42:40 | announced | |
Publication List
Keyword List
| submitter keyword: Protease cleavage assay,Thermolysin, iTRAQ |
Contact List
| Sigurd Wilbanks |
|---|
| contact affiliation | Department of Biochemistry, University of Otago, New Zealand |
| contact email | sigurd.wilbanks@otago.ac.nz |
| lab head | |
| Torsten Kleffmann |
|---|
| contact affiliation | Department of Biochemistry, University of Otago |
| contact email | torsten.kleffmann@otago.ac.nz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064530
- Label: PRIDE project
- Name: Cleavage preference of metalloprotease EA1
