PXD064529-1

PXD064529 is an original dataset announced via ProteomeXchange.

Title	Paracrine Signals from HIV-1 Infected Immune Cells Reprogram Cervical Cancer Pathways
Description	Co-infection of human papillomavirus (HPV) and human immunodeficiency virus type 1 (HIV-1) in women have a six-fold higher risk of developing cervical cancer compared to those without HIV. To evaluate how paracrine signals from HIV-infected T-cells remodeled the proteome of cervical epithelial cells in culture, primary CD4+ T cells isolated from PBMC-enriched leukapheresis products (leukopaks) from two healthy donors were infected or uninfected with a replication-competent pNL4-3 HIV-1 strain for 72 hours. Secretome from the CD4+ T cell cultures was used to stimulate the human HPV-negative cervical epithelial cell line, C33A, for 72 hours. Then, C33A cells were harvested, and cell lysates were digested and subjected to global quantitative mass spectrometry (MS) based abundance proteomics and phosphoproteomics analyses. Both proteomics and phosphoproteomics outputs were analysed using bioinformatics approaches. These datasets revealed altered expression of proteins in the MAPK, PI3K-AKT, and β-catenin signaling pathways. Additionally, MS phosphoproteomics analysis confirmed PI3K-AKT pathway activation in cervical cells exposed to conditioned media from HIV-1-infected T cells.
HostingRepository	PRIDE
AnnounceDate	2026-06-25
AnnouncementXML	Submission_2026-06-25_15:55:09.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehdi Bouhaddou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-02 14:36:29	ID requested
⏵ 1	2026-06-25 15:55:10	announced

Olwal CO, Rathore U, Makanani SK, Kaushal P, Ashley IA, Ummadi MR, Appiah V, Djomkam Zune AL, Blanc SF, Winters DM, Delgado Y, Muthoka K, Fabius JM, Eckhardt M, Kaake RM, Su M, Fregoso OI, Hultquist JF, Orang'o EO, Swaney DL, Kyei GB, Krogan NJ, Quashie PK, Bediako Y, Bouhaddou M, Paracrine signals from HIV-1-infected immune cells reprogram cervical cancer pathways. iScience, 29(6):116083(2026) [pubmed]

10.1016/j.isci.2026.116083;

submitter keyword: HPV/HIV coinfection, HPV-associated cervical cancer, MAPK and PI3K signaling, Network propagation

Mehdi Bouhaddou
contact affiliation	1Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA 2Institute for Quantitative and Computational Biosciences, University of California, Los Angeles, Los Angeles, CA, USA 3Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA
contact email	bouhaddoulab@gmail.com
lab head
Mehdi Bouhaddou
contact affiliation	UCLA
contact email	bouhaddoulab@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064529

PRIDE project URI

• PRIDE
  1. PXD064529
     1. Label: PRIDE project
     2. Name: Paracrine Signals from HIV-1 Infected Immune Cells Reprogram Cervical Cancer Pathways