PXD064514 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sex-specific Phosphoproteome Responses to Calorie Restriction and Insulin in Skeletal Muscle from Older Rats |
| Description | Aging impairs skeletal muscle insulin sensitivity, yet the underlying molecular mechanisms remain incompletely understood. Here, we examined how short-term caloric restriction (CR) alters muscle insulin action and signaling in aged rats (22 months old) of both sexes. After 8 weeks of CR or ad libitum feeding, we assessed insulin-stimulated glucose uptake (ISGU) ex vivo and performed deep phosphoproteomic profiling. CR enhanced ISGU in both sexes, with a more pronounced effect in females. Insulin-regulated phosphoproteomic responses were concordant between males and females, yet females exhibited more robust and widespread phosphorylation changes, potentially explaining their greater ISGU. CR induced extensive remodeling of basal muscle phosphorylation, particularly within structural and contractile pathways. Personalized phosphoproteomic analysis identified insulin-responsive sites on Leiomodin-1 (Lmod1) and EH domain-binding protein 1-like protein 1 (Ehbp1l1) that correlated with ISGU across individuals. Notably, Lmod1demonstrated strong genetic association with glycemic traits in humans, and several regulated sites overlapped with phosphosites observed in human clamp studies, reinforcing their translational relevance. Collectively, these findings reveal sex-dependent and diet-sensitive phosphosignaling mechanisms that modulate muscle insulin responsiveness during aging and nominate candidate regulatory nodes for future study. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-24 |
| AnnouncementXML | Submission_2025-11-24_12:11:43.662.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sean Humphrey |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-02 07:25:20 | ID requested | |
| ⏵ 1 | 2025-11-24 12:11:44 | announced | |
Publication List
| Wang H, Madsen S, Needham EJ, Humphrey SJ, Zheng A, Arias EB, St, รถ, ckli J, Cutler HB, James DE, Cartee GD, Sex-specific phosphoproteome responses to calorie restriction and insulin in skeletal muscle from older rats. J Gerontol A Biol Sci Med Sci, 80(12):(2025) [pubmed] |
| 10.1093/gerona/glaf231; |
Keyword List
| submitter keyword: calorie restriction, phosphoproteome, rats, skeletal muscle, muscle |
Contact List
| Sean Humphrey |
|---|
| contact affiliation | Functional Phosphoproteomics lab, Murdoch Children's Research Institute, Melbourne Australia |
| contact email | sean.humphrey@mcri.edu.au |
| lab head | |
| Sean Humphrey |
|---|
| contact affiliation | Murdoch Children's Research Institute |
| contact email | sean.j.humphrey@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064514
- Label: PRIDE project
- Name: Sex-specific Phosphoproteome Responses to Calorie Restriction and Insulin in Skeletal Muscle from Older Rats
