PXD064487-1

PXD064487 is an original dataset announced via ProteomeXchange.

Title	A simplified perchloric acid workflow with neutralization (PCA-N) for democratizing deep plasma proteomics at population scale - Submission04
Description	This project presents PCA-N, a novel plasma proteomics workflow that uses perchloric acid with neutralization to dramatically improve sample preparation efficiency. By eliminating solid-phase extraction and enabling 384-well plate compatibility, PCA-N reduces sample volume requirements to just 5 μL, cuts costs to under $1 per sample, and allows processing of up to 13,000 samples daily. Validated on over 34,000 samples across 311 days, this method doubles proteome depth compared to conventional approaches while maintaining robust measurement precision, making large-scale plasma proteomics studies feasible for biomarker discovery and precision medicine.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-06_12:21:58.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-01 03:55:30	ID requested
⏵ 1	2025-10-06 12:21:59	announced

Dataset with its publication pending

submitter keyword: LC-MSMS,Plasma

Prof. Dr. Matthias Mann
contact affiliation	Dept. Proteomics and Signal Transduction Director at the Max-Planck Institute of Biochemistry
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064487

PRIDE project URI

• PRIDE
  1. PXD064487
     1. Label: PRIDE project
     2. Name: A simplified perchloric acid workflow with neutralization (PCA-N) for democratizing deep plasma proteomics at population scale - Submission04