PXD064476 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deciphering the dynamic proteome of multiciliated cells |
| Description | Multiciliated cells (MCCs) are essential for generating directional fluid flow across specialized epithelia in various vertebrate organs. MCC differentiation involves a unique, tightly regulated program characterized by massive centriole amplification, independently of DNA replication. Although much is known about the transcriptional control of MCC development, insights into proteome dynamics have been limited due to the lack of suitable models. In this study, we report the generation of a stable inducible MCC line, derived from Xenopus laevis A6 kidney epithelial cells. Upon induction of the MCC master regulator Multicilin (MCI), most A6-MCI cells synchronously differentiate into mature MCCs in 48 hours. Using this novel resource, custom antibodies and super-resolution imaging, we could characterize Xenopus deuterosomes, the platforms that allow massive centriole synthesis in vertebrate MCCs. We performed a detailed proteomic profiling throughout MCC differentiation, and uncovered previously uncharacterized regulators. Notably, we highlight a critical role for CDK7 in MCC differentiation in both Xenopus and human systems. Our work provides a valuable resource for mechanistic studies of MCC biology and opens avenues to identify novel therapeutic targets for motile ciliopathies. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-06 |
| AnnouncementXML | Submission_2026-02-06_14:12:22.475.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | AUDEBERT Stephane |
| SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: NEWT:8355; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-31 09:57:11 | ID requested | |
| ⏵ 1 | 2026-02-06 14:12:22 | announced | |
Publication List
Keyword List
| submitter keyword: LC-MSMS,multiciliated cells proteome (MCC), deuterosome, centriole, xenopus |
Contact List
| Audebert Stephane PhD, |
|---|
| contact affiliation | Marseille Proteomic, MaP-CRCM Centre de Recherche en Cancérologie de Marseille, CRCM Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France |
| contact email | stephane.audebert@inserm.fr |
| lab head | |
| AUDEBERT Stephane |
|---|
| contact affiliation | Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France |
| contact email | stephane.audebert@inserm.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064476 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064476
- Label: PRIDE project
- Name: Deciphering the dynamic proteome of multiciliated cells
