PXD064459-1

PXD064459 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	The ERVK3-1 microprotein interacts with the HUSH complex
Description	Human endogenous retroviruses (hERVs) are molecular remnants of ancient retroviruses that integrated into the human germline and now make up 8% of the human genome. The human ERVK3-1 locus was recently annotated as encoding a 109-amino acid microprotein homologous to a retroviral Rec domain. ERVK3-1 is ubiquitously expressed in normal human cells and tissues. However, because this locus was thought to be non-coding until recently, it is currently unknown whether the ERVK3-1 microprotein has a function in human cells. We demonstrate that the ERVK3-1 microprotein interacts with PPHLN1, a component of the HUSH complex, and contributes to target gene repression. We thus suggest that the ERVK3-1 Rec protein may have been co-opted by human cells for molecular sensing and/or feedback regulation of hERV expression.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:27:03.658.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064459
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-30 12:47:51	ID requested
⏵ 1	2025-08-24 16:27:04	announced

Publication List

10.1021/acs.biochem.5c00023;
Jayatissa A, Jaunbocus N, Erkalo B, Jiang K, Zheng SJ, Su H, Yan L, Choi JY, Vaughan J, Bacchiocchi A, Na Z, Cao X, Halaban R, Saghatelian A, Craft J, Chen YG, Slavoff SA, The ERVK3-1 Microprotein Interacts with the HUSH Complex. Biochemistry, 64(15):3372-3381(2025) [pubmed]
10.6019/PXD064459;

10.1021/acs.biochem.5c00023;

Jayatissa A, Jaunbocus N, Erkalo B, Jiang K, Zheng SJ, Su H, Yan L, Choi JY, Vaughan J, Bacchiocchi A, Na Z, Cao X, Halaban R, Saghatelian A, Craft J, Chen YG, Slavoff SA, The ERVK3-1 Microprotein Interacts with the HUSH Complex. Biochemistry, 64(15):3372-3381(2025) [pubmed]

10.6019/PXD064459;

Keyword List

submitter keyword: hERVs, proximity labelling,LC-MSMS

submitter keyword: hERVs, proximity labelling,LC-MSMS

Contact List

Sarah Slavoff
contact affiliation	Yale University Institute for Biomolecular Design and Discovery, West Haven, CT 06516 Yale University Department of Chemistry, New Haven, CT 06520 Yale University Department of Biophysics and Biochemistry, New Haven, CT 06520
contact email	sarah.slavoff@yale.edu
lab head
Kevin Jiang
contact affiliation	Yale University
contact email	kevin.jiang.kj447@yale.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD064459
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD064459

PRIDE project URI

Repository Record List

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