PXD064453-1

PXD064453 is an original dataset announced via ProteomeXchange.

Title	Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts (crosslinking data)
Description	The TOC protein translocon delivers thousands of nucleus-encoded proteins to chloroplasts and to related non-photosynthetic plastids. It comprises the β-barrel channel, Toc75, and multiple isoforms of receptor GTPases, Toc33 and Toc159. However, exactly how TOC complexes are assembled in different plastid types, is not known. Here, we present detailed characterization of two distinct TOC complexes, TOC-P and TOC-N, from photosynthetic chloroplasts and non-photosynthetic plastids, respectively. The assembled complexes are distinguished by having different sets of receptors, but both possess Toc75 which we identify as a central hub in TOC biogenesis: assembly is driven by TOC75 expression, with the Toc33and Toc159 GTPases being added sequentially thereafter. Integrative structural analysis revealed a modular architecture for TOC-P comprising a cytosolic GTPase receptor module linked flexibly to a membrane β-barrel channel module. TOC-N has a similar overall architecture, albeit with some clear differences that likely account for observed functional differences related to client specificity.
HostingRepository	PRIDE
AnnounceDate	2026-03-22
AnnouncementXML	Submission_2026-03-22_15:49:27.701.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaishnavi Ravikumar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-30 08:58:15	ID requested
⏵ 1	2026-03-22 15:49:28	announced

Dataset with its publication pending

submitter keyword: None

Marjorie Fournier
contact affiliation	Deaprtment of Biochemistry, University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
lab head
Vaishnavi Ravikumar
contact affiliation	Dept. of Biochemistry, University of Oxford
contact email	vaishnavi.ravikumar@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064453

PRIDE project URI

• PRIDE
  1. PXD064453
     1. Label: PRIDE project
     2. Name: Assembly of two functionally-distinct protein import complexes in the outer membrane of plant chloroplasts (crosslinking data)