PXD064428 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Production of isoprenol using the IPP-bypass pathway in Rhodosporidium toruloides |
| Description | There is increasing demand for domestically generated-bioderived aviation fuels to strengthen the national energy supply while also reducing carbon emissions. Developing these supply chains provides an opportunity to explore novel aviation fuel components, such as 1,4-dimethylcyclooctane (DMCO). In this work we explore the use of a promising bioproduction yeast, Rhodospridium toruloides, to generate isoprenol, a chemical intermediate of DMCO. We first introduced the isoprenol production pathways most successful in E. coli and S. cerevisiae and determined the IPP-bypass pathway was a promising route for isoprenol production in R. toruloides. We next demonstrated that increasing flux through the mevalonate pathway increased isoprenol production. Through proteomics we identified a potential bottleneck in the expression of the final phosphatase in the IPP-bypass pathway. We therefore explored alternatives to increase expression by screening a selection of phosphatases for isoprenol production in R. toruloides. Finally, the top three strains from all screens were evaluated for production in sorghum hydrolysates generated using choloinium lysonate. Through this work the top producing strain generated up to 93.1 mg/L in mock medium and 85.5 mg/L in sorghum hydrolysates. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-09 |
| AnnouncementXML | Submission_2026-04-09_12:38:47.833.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Christopher Petzold |
| SpeciesList | scientific name: Rhodotorula toruloides; NCBI TaxID: NEWT:5286; |
| ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-29 13:30:19 | ID requested | |
| ⏵ 1 | 2026-04-09 12:38:48 | announced | |
Publication List
| 10.1186/s13068-026-02750-w; |
| Garcia VE, Geiselman GM, Hwang HJ, Chen Y, Gin JW, McFadden-Mitchell J, Montgomery V, Kakumanu R, Goswami S, McCauley J, Adamczyk PA, Baidoo EEK, Petzold CJ, Simmons BA, Lee TS, Gladden JM, Evaluating isoprenol production using the IPP-bypass pathway in the oleaginous yeast Rhodosporidium toruloides. Biotechnol Biofuels Bioprod, 19(1):(2026) [pubmed] |
Keyword List
| submitter keyword: Isoprenol |
| Rhodosporidium toruloides |
| IPP-Bypass pathway |
| Phosphatase |
| Advanced Biofuel |
| Sustainable Aviation Fuel;JBEI |
Contact List
| Christopher Petzold |
|---|
| contact affiliation | Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720 |
| contact email | cjpetzold@lbl.gov |
| lab head | |
| Christopher Petzold |
|---|
| contact affiliation | Lawrence Berkeley National Laboratory |
| contact email | cjpetzold@lbl.gov |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064428
- Label: PRIDE project
- Name: Production of isoprenol using the IPP-bypass pathway in Rhodosporidium toruloides
