PXD064417 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Decontamination of archaeological dental enamel for palaeoproteomics |
| Description | Archaeological materials will accumulate contamination from sources such as handling and storage, which may affect downstream analyses. For dental enamel, it has often been assumed that removal of contamination is not necessary prior to palaeoproteomic analyses, due to the extraction protocols that are used, and relatively straightforward bioinformatic separation of contaminants and endogenous proteins. Here, we artificially contaminate Pleistocene Rhinoceros dental enamel with modern dog saliva, in order to evaluate the effects of contamination and to compare decontamination protocols. We find that although the contaminating proteins cannot be identified, contamination does lead to a significant loss of endogenous proteomic data. A comparison of published decontamination methods thereafter shows that a wash with water or bleach is most efficient at removing contamination. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-15 |
| AnnouncementXML | Submission_2025-12-15_02:39:29.733.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zandra Fagernäs |
| SpeciesList | scientific name: Coelodonta antiquitatis (woolly rhinoceros); NCBI TaxID: NEWT:222863; |
| ModificationList | hydroxylated proline; phosphorylated residue; deamidated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-29 07:58:27 | ID requested | |
| ⏵ 1 | 2025-12-15 02:39:30 | announced | |
Publication List
Keyword List
| submitter keyword: Dental enamel, Contamination, Archaeology, Pleistocene, Palaeoproteomics |
Contact List
| Frido Welker |
|---|
| contact affiliation | Globe Institute, University of Copenhagen, Denmark |
| contact email | frido.welker@sund.ku.dk |
| lab head | |
| Zandra Fagernäs |
|---|
| contact affiliation | Globe Institute, University of Copenhagen |
| contact email | zandra.fagernas@sund.ku.dk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD064417 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064417
- Label: PRIDE project
- Name: Decontamination of archaeological dental enamel for palaeoproteomics
