PXD064364-1

PXD064364 is an original dataset announced via ProteomeXchange.

Title	ChAHP-mediated Chromatin Remodeling Activity Restricts Transcription Factor Access to chromatin
Description	Transcriptional control in eukaryotes relies on the coordinated action of chromatin-based regulatory mechanisms, including control of nucleosome occupancy and chromatin modifications. We previously identified the ChAHP complex – comprising the chromatin remodeler CHD4, transcription factor ADNP, and HP1 proteins – as a site-specific transcriptional regulator and antagonist of CTCF engagement with chromatin. However, the molecular basis of these functions remained unresolved. Here, we show that ChAHP-associated CHD4 is essential for transposon repression, while HP1 proteins are dispensable. We further demonstrate that CHD4’s remodeling activity is not required for chromatin binding, but is critical for both retrotransposon silencing and efficient CTCF antagonism. We propose ADNP is a sequence-specific recruiter of chromatin remodeling activity, facilitating transcriptional control and localized modulation of chromatin architecture.
HostingRepository	PRIDE
AnnounceDate	2026-07-17
AnnouncementXML	Submission_2026-07-17_02:30:41.559.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Seebacher
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-28 02:46:21	ID requested
⏵ 1	2026-07-17 02:30:42	announced

Dataset with its publication pending

submitter keyword: mouse ESC,ChAHP, SINE, CTCF, CHD4, IP-MS, MaxQuant, retrotransposon control, Orbitrap Fusion Lumos, einprot

Marc Buehler
contact affiliation	Genome Regulation, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
contact email	marc.buehler@fmi.ch
lab head
Jan Seebacher
contact affiliation	FMI Basel
contact email	jan.seebacher@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD064364

PRIDE project URI

• PRIDE
  1. PXD064364
     1. Label: PRIDE project
     2. Name: ChAHP-mediated Chromatin Remodeling Activity Restricts Transcription Factor Access to chromatin