PXD064360-1

PXD064360 is an original dataset announced via ProteomeXchange.

Title	RNA binding regulation is a new dimension in the type I IFN response
Description	The type I interferon (IFN-I) response shapes the intracellular environment to supress virus infection. Historically, this remodelling has been linked to the transcriptional induction of interferon stimulated genes (ISGs). However, IFN-I-driven post-translational regulation of proteins already present in the cell remains relatively unexplored. Here, we profiled the activity of cellular RNA-binding proteins (RBPs), which are key players in antiviral immunity. Using RNA interactome capture (RIC), we identified hundreds of RBPs whose association with RNA is regulated by IFN-I (IR-RBPs). Among these IR-RBPs are both known antiviral proteins and novel candidates identified here through a knockdown screen. By modifying RIC to study IR-RBPs' phosphorylation states, we identified several putative instances of IFN-I-driven phospho-regulation of RNA binding. We experimentally confirmed this phospho-driven regulation for MATR3. Altogether, our results reveal a new dimension of the cell’s antiviral programme, in which the cellular RNA-bound proteome is remodelled by IFN-I.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-23_07:41:08.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louisa Iselin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-28 02:26:08	ID requested
⏵ 1	2026-02-23 07:41:08	announced

Dataset with its publication pending

submitter keyword: host-virus interactions,RNA, interferon, phosphorylation, antiviral factors, phosphoproteomics, RNA-binding protein, RNA interactome, RBP

Alfredo Castello
contact affiliation	MRC University of Glasgow Centre for Virus Research
contact email	alfredo.castello@glasgow.ed.ac.uk
lab head
Louisa Iselin
contact affiliation	University of Glasgow
contact email	iselin.louie@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064360

PRIDE project URI

• PRIDE
  1. PXD064360
     1. Label: PRIDE project
     2. Name: RNA binding regulation is a new dimension in the type I IFN response