PXD064324-1

PXD064324 is an original dataset announced via ProteomeXchange.

Title	Early microglia progenitors colonize the embryonic CNS via integrin-mediated transmigration from the pial surface
Description	Microglia progenitors initially colonize the developing central nervous system (CNS) around embryonic day (E) 9.5 in the mouse. Even though their ontogeny from early erythromyeloid progenitors in the yolk sac is well defined, the molecular machinery needed to enter the developing CNS is so far not resolved. Here, we combined unbiased transcriptomic and proteomic approaches to screen for potential factors. We identified a distinct and dynamic integrin expression profile on microglial progenitors, whereas in parallel the developing CNS peaks in the production of extracellular matrix (ECM) molecules. We found that this ECM is deposited along the pial surface between CNS and mesenchyme, where microglial progenitors interact with deposited ECM along the barrier zone of the CNS, pointing to a mesenchyme-to-CNS transmigration route of microglial progenitors. Loss of the integrin adaptor molecule Tln1 in microglia progenitors led to a reduced capacity to enter the developing CNS, whereas their numbers in the surrounding mesenchyme remained unchanged. However, deficiency of Itgb1 or Itgb2 alone was not sufficient to affect this migration. Our data revealed an essential role of a complex integrin expression profile on microglia progenitors to equip these cells to interact with ECM at the pial surface of the developing CNS and facilitate their entrance from the surrounding mesenchyme.
HostingRepository	MassIVE
AnnounceDate	2025-05-27
AnnouncementXML	Submission_2025-05-27_02:14:37.703.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Schilling
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; TMT6plex
Instrument	instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-27 01:40:23	ID requested
⏵ 1	2025-05-27 02:14:38	announced

no publication

submitter keyword: microglia, microglia development, microglia progenitor recruitment, macrophage progenitor migration, integrins, DatasetType:Proteomics

Prof. Dr. Oliver Schilling
contact affiliation	Medical Center - University of Freiburg
contact email	oliver.schilling@uniklinik-freiburg.de
lab head
Oliver Schilling
contact affiliation	University of Freiburg
contact email	oliver.schilling@mol-med.uni-freiburg.de
dataset submitter

MassIVE dataset URI

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