PXD064257-1

PXD064257 is an original dataset announced via ProteomeXchange.

Title	The TFIID complex is a new autoantibody target in systemic sclerosis.
Description	Systemic sclerosis (SSc) is characterized by systemic fibrosis, vascular dysfunction, and the presence of antinuclear autoantibodies. In up to 10% of persons with SSc, the target antigens of the antinuclear autoantibodies are unknown. Using IP-MS, we here describe another new target antigen in SSc. We examined a database of IP-MS identifications of 106 persons with SSc from two Belgian academic hospitals [Ghent University Hospital (n=59) and University Hospitals Leuven (n=47)] without autoantibody specificity as tested by commercial assays.TATA-binding protein (TBP)-associated factors (TAFs) were precipitated by sera of four persons (two from Ghent, two from Leuven). Together with TBP, TAFs form the three-lobed transcription factor IID complex (TFIID) (figure 1B). The TFIID complex recognizes core promoter sequences on DNA. The binding of TFIID is the first step of the assembly of the preinitiation complex, which is needed to position RNA polymerase II onto the correct DNA sequence to start transcription. To identify additional cases, we tested samples from individuals with SSc with unidentified autoantibodies from 3 extra hospitals [Cliniques Universitaires Saint-Luc (Belgium) (n=45), Chris Hani Baragwanath Hospital (South Africa) (n=28) and Centre Hospitalier Universitaire d’Angers (France) (n=13)] by IP-MS. Two additional cases precipitating TFIID subunits were identified. In order to confirm the specificity of anti-TIFIID antibodies for SSc, IP-MS was performed in healthy individuals (n=34) and in patients with idiopathic inflammatory myopathy (IIM, n=12), Sjögren’s disease (SjD, n=12), systemic lupus erythematosus (SLE, n=36) and anti-topoisomerase-I-, anti-centromere- or anti-RNA polymerase III-positive SSc (n=28). TAF9, TAF9B and TAF10 were often precipitated in these control samples. In conclusion, we describe TFIID as a new target of autoantibodies in SSc, thereby expanding the spectrum of SSc-associated autoantigens related to transcription.
HostingRepository	PRIDE
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-02_01:25:40.025.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064257
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Birthe Michiels
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Xevo G3 QTof; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 22:49:54	ID requested
⏵ 1	2025-07-02 01:25:40	announced

10.1016/j.ard.2025.05.008;

10.6019/PXD064257;

Vulsteke JB, Michiels B, Smith V, Piette Y, Vanthuyne M, Lacout C, Brochard A, Tikly M, De Leeuw J, Dillaerts D, Dehaemers T, De Haes P, Lenaerts JL, Blockmans D, McHugh N, Tansley S, Vinatier E, Houssiau F, Bonroy C, De Langhe E, Bossuyt X, The TFIID complex is a new autoantibody target in systemic sclerosis. Ann Rheum Dis, ():(2025) [pubmed]

submitter keyword: anti-nuclear autoantibodies,Systemic sclerosis, IP-MS, TFIID, ANA

Xavier Bossuyt
contact affiliation	Clinical and Diagnostic Immunology, Department Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium
contact email	xavier.bossuyt@uzleuven.be
lab head
Birthe Michiels
contact affiliation	KU Leuven, Clinical and Diagnostic Immunology, Leuven, Belgium
contact email	birthe.michiels@uzleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064257
     1. Label: PRIDE project
     2. Name: The TFIID complex is a new autoantibody target in systemic sclerosis.