PXD064255-1

PXD064255 is an original dataset announced via ProteomeXchange.

Title	TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin
Description	TEA domain transcription factor 1 (TEAD1), a Hippo pathway transcription factor important in cellular homeostasis and development, is increasingly implicated in cancer biology. Here, we reveal a novel role for TEAD1 in organizing nuclear condensates, independent of active transcription. Using high-resolution imaging, ChIP-seq, RNA-seq and proximity-based proteomics, we demonstrate that in patient-derived renal cell carcinoma cells, TEAD1 forms micron-sized foci by binding to the heterochromatic pericentromeric regions using its DNA-binding domain. These TEAD1 foci do not mediate transcription but instead serve as depots for excess TEAD1. This contrasts with TEAD1 organization in other genomic regions of both RCC and normal kidney cells, where TEAD1 associates with markers of active transcription. Our findings provide a mechanistic framework for TEAD1’s dual regulatory roles, offering new insights into its contribution to transcriptional dysregulation and tumor progression.
HostingRepository	PRIDE
AnnounceDate	2025-06-02
AnnouncementXML	Submission_2025-06-02_08:17:22.653.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064255
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Danfeng Cai
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 18:25:01	ID requested
⏵ 1	2025-06-02 08:17:23	announced

10.6019/PXD064255;

submitter keyword: Human, Biotin, Kidney

Danfeng Cai
contact affiliation	Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA; Department of Oncology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
contact email	danfeng.cai@jhu.edu
lab head
Danfeng Cai
contact affiliation	Johns Hopkins Bloomberg School of Public Health
contact email	danfeng.cai@jhu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064255
     1. Label: PRIDE project
     2. Name: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin