PXD064226-1

PXD064226 is an original dataset announced via ProteomeXchange.

Title	The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii
Description	Toxoplasma gondii is a significant pathogen affecting both humans and animals, with clinical symptoms primarily driven by the uncontrolled proliferation of tachyzoites. In this study, we reveal the essential role and functional plasticity of the PP2A-2 holoenzyme in orchestrating daughter cell emergence during tachyzoite division. The holoenzyme, composed of the regulatory subunit TgPR48 (PP2A-B2), the catalytic subunit PP2A-C2, and the scaffolding subunit PP2A-A2, is critical for successful cell division. Depletion of any of these subunits resulted in severe defects in daughter cell emergence and impaired proper cell separation. Phosphoproteomic analysis following PP2A-C2 depletion identified multiple differentially phosphorylated proteins, including potential regulatory substrates DCS1 and DCS2. However, mutating several phosphorylation sites on DCS1 and DCS2 did not significantly alter their function. Interestingly, depletion of DCS1 or DCS2 disrupted TgPR48 localization, while PR48 overexpression partially rescued defects in DCS2-depleted parasites but not in DCS1-depleted parasites. This suggests that PP2A-2 may compensate through additional, yet unidentified, substrates. Our findings highlight the crucial role of PP2A-2–mediated dephosphorylation in T. gondii tachyzoite division and identify potential molecular targets for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_08:32:17.069.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JinLei Wang
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	phosphorylated residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 01:24:07	ID requested
⏵ 1	2025-08-21 08:32:17	announced

Dataset with its publication pending

submitter keyword: Toxoplasma gondii

PP2A-2 phosphatase

Cytokinesis

Cell division

Daughter cell emergence

Phosphoproteomics

XingQuan Zhu
contact affiliation	State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China
contact email	xingquanzhu1@hotmail.com
lab head
JinLei Wang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
contact email	wangjinlei90@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064226
     1. Label: PRIDE project
     2. Name: The PP2A-2 holoenzyme orchestrates daughter cell emergence during cytokinesis in Toxoplasma gondii