PXD064203-1

PXD064203 is an original dataset announced via ProteomeXchange.

Title	A protein adaptor mediating Ap4A-dependent control of protein acetylation.
Description	Reversible lysine acetylation is a highly conserved post-translational modification across all domains of life, modulating diverse cellular processes. However, the regulatory mechanisms governing this modification remain poorly understood. Here, we uncover a novel regulatory system in Bacillus subtilis involving the histone deacetylase (HDAC)-like protein AcuC, which targets multiple substrates, including acetyl-CoA synthetase and translation elongation factor. We show that AcuC is inhibited through complex formation with AcuB, a previously uncharacterized protein. We further demonstrate that the alarmone diadenosine tetraphosphate (Ap4A) binds to the cystathionine beta-synthase (CBS) domain of AcuB, stabilizing the AcuB-AcuC complex and enhancing AcuC inhibition. This study identify AcuB as an Ap4A-enhanced deacetylation inhibitor, revealing a new regulatory mechanism for HDAC-like proteins. Our findings also provide the first direct evidence that the secondary messenger Ap4A modulates protein (de)acetylation, highlighting a regulatory network linking cell stress, proteome acetylation, and acetyl-CoA availability. These insights advance our understanding of protein acetylation control.
HostingRepository	PRIDE
AnnounceDate	2026-01-21
AnnouncementXML	Submission_2026-01-21_08:04:26.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieland Steinchen
SpeciesList	scientific name: Geobacillus thermodenitrificans NG80-2; NCBI TaxID: NEWT:420246;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 11:06:23	ID requested
⏵ 1	2026-01-21 08:04:27	announced

Dataset with its publication pending

ProteomeXchange project tag: Hydrogen Deuterium Exchange (HDX-MS)

submitter keyword: Lysine acetylation, CBS, HDX-MS, Ap4A

Gert Bange
contact affiliation	1 Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 14, 35043 Marburg, Germany 2 University of Marburg, Center for Synthetic Microbiology (SYNMIKRO) & Departments of Chemistry and Biology, Karl-von-Frisch Strasse 14, 35043 Marburg, Germany
contact email	gert.bange@synmikro.uni-marburg.de
lab head
Wieland Steinchen
contact affiliation	Philipps-University Marburg, Department of Chemistry & SYNMIKRO, Karl-von-Frisch-Straße 14, 35043 Marburg, GERMANY
contact email	wieland.steinchen@synmikro.uni-marburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD064203

PRIDE project URI

• PRIDE
  1. PXD064203
     1. Label: PRIDE project
     2. Name: A protein adaptor mediating Ap4A-dependent control of protein acetylation.