PXD064195-1

PXD064195 is an original dataset announced via ProteomeXchange.

Title	A H2O2-Free Proximity Proteomics Technology for Exploring Dynamic Protein Complexes in Living Systems
Description	Dynamic protein complex assembly is critical for regulating various biological processes. Proximity labeling(PL), best represented by the ascorbate peroxidase APEX2, allows these molecular events to be captured in living cells in a spatiotemporal manner. However, the hydrogen peroxide (H2O2) dependence of APEX2 has hindered its application in sensitive living systems. Here, we introduce ROProx, a radical- and oxygen-driven photoreactive PL technologythat leverages the chemically evolved biotin-naphthylamine probe BN2, which has strong binding affinity for APEX2, and the unexpected tyrosyl radicals in APEX2. ROProx labels dynamic cytosolic protein complexes in living cells within seconds, with a range of 10 nm and is precisely controlled by mild blue light irradiation without H2O2. Additionally, we applied ROProx to explore the phosphotyrosine29 dependent GRB2 interactome in living mice by simply injecting BN2 for 5 minutes. ROProx should therefore open broad opportunities for PL chemical evolution and applications in other living systems.
HostingRepository	PRIDE
AnnounceDate	2026-04-01
AnnouncementXML	Submission_2026-04-01_08:42:44.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ke Mi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 08:17:07	ID requested
⏵ 1	2026-04-01 08:42:45	announced

Dataset with its publication pending

submitter keyword: ascorbate peroxidase, tyrosine radical, H2O2-free proximity labeling

Ruijun tian
contact affiliation	Department of Chemistry, Southern University of Science and Technology
contact email	tianrj@sustech.edu.cn
lab head
Ke Mi
contact affiliation	P.h.d student
contact email	11930781@mail.sustech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD064195

PRIDE project URI

• PRIDE
  1. PXD064195
     1. Label: PRIDE project
     2. Name: A H2O2-Free Proximity Proteomics Technology for Exploring Dynamic Protein Complexes in Living Systems