PXD064190-1

PXD064190 is an original dataset announced via ProteomeXchange.

Title	Sex Differences in Drosophila Sexually Dimorphic Infection Outcome
Description	Sexual dimorphism in infection outcomes is a pervasive phenomenon, the underlying mechanisms of which remain incompletely understood. Here, utilizing Pseudomonas entomophila intestinal infection in Drosophila, we demonstrated that sex differences in intestinal redox processes contribute to female bias in susceptibility to gut infection. Female inability to overcome excessive pathogen-induced oxidative stress results in defecation blockage, pathogen persistence, and host death. Male flies exhibit increased carbohydrate metabolism and pentose phosphate pathway activity – a key antioxidant defense system. This allows males to withstand oxidative stress-induced defecation blockage and clear the pathogen from the intestine, resulting in survival. Additionally, P. entomophila showed increased expression of several virulence factors, including RNA-binding protein Hfq, in the female gut, contributing to female-biased virulence of P. entomophila. Thus, the effect of the gut metabolic environment on host defenses and pathogen virulence determines the sex differences in intestinal infection outcomes.
HostingRepository	PRIDE
AnnounceDate	2026-06-10
AnnouncementXML	Submission_2026-06-10_03:49:26.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marko Rubinic
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; scientific name: Pseudomonas entomophila L48; NCBI TaxID: NEWT:384676;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 05:48:45	ID requested
⏵ 1	2026-06-10 03:49:27	announced

Dataset with its publication pending

submitter keyword: Gut Infection, Pseudomonas, Drosophila melanogaster, Sexual Dimorphism

Igor Iatsenko
contact affiliation	Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany
contact email	iatsenko@mpiib-berlin.mpg.de
lab head
Marko Rubinic
contact affiliation	Research group Genetics of host-microbe interactions, Max Planck Institute for Infection Biology, Charitéplatz 1, 10117 Berlin, Germany
contact email	rubinic@mpiib-berlin.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064190

PRIDE project URI

• PRIDE
  1. PXD064190
     1. Label: PRIDE project
     2. Name: Sex Differences in Drosophila Sexually Dimorphic Infection Outcome