PXD064189-1

PXD064189 is an original dataset announced via ProteomeXchange.

Title	E. faecium response of ATP-binding protein activity to human serum and colon organoid extract
Description	Enterococcus faecium has emerged as a major nosocomial and opportunistic pathogen, increasingly associated with severe infections such as urinary tract infections, bacteraemia, and endocarditis. Notably, it exhibits intrinsic resistance to many antibiotics and has a remarkable capacity to acquire additional resistance mechanisms, making it particularly difficult to treat—especially due to its heightened resistance to vancomycin and ampicillin. To address these therapeutic challenges, we employed a chemical proteomics strategy to investigate ATP-binding protein activity in E. faecium. Using the activity-based probe (ABP) desthiobiotin-ATP, we profiled protein activity under different growth conditions, including Brain-Heart Infusion (BHI) broth supplemented with either human serum or colon organoid extract. Our analysis identified 230 enriched putative ATP-binding proteins. Of these, 31 proteins were specifically activated in response to the organoid extract, and 19 in response to human serum. Notably, the ABC transporter TcyA and the histidine kinase ArlS were activated in the presence of the organoid extract. These findings suggest that E. faecium dynamically modulates its metabolic and regulatory pathways in response to host-derived signals from human serum and organoid effluent, potentially enhancing its adaptability and pathogenicity in various host environments. We highlight TcyA and ArlS for their known roles in virulence in other pathogens, making them promising targets for further functional studies in E. faecium.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:24:32.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeanette, Slettnes Grunnvåg
SpeciesList	scientific name: Enterococcus faecium; NCBI TaxID: NEWT:1352;
ModificationList	biotinylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 05:40:23	ID requested
⏵ 1	2026-09-06 16:24:33	announced

10.1093/femsmc/xtag038;

Grunnv, å, g JS, Hegstad K, Dela Ahator S, Lentz CS, in response to host-derived cues. FEMS Microbes, 7():xtag038(2026) [pubmed]

submitter keyword: activity-based protein profiling, desthiobiotin-ATP, ABC transporters, kinases

Christian S. Lentz
contact affiliation	Department of Medical Biology, Health Faculty, UiT - The Arctic University of Norway, Norway.
contact email	christian.s.lentz@uit.no
lab head
Jeanette, Slettnes Grunnvåg
contact affiliation	UiT - The Arctic University of Norway
contact email	jeanette.grunnvag@uit.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064189
     1. Label: PRIDE project
     2. Name: E. faecium response of ATP-binding protein activity to human serum and colon organoid extract