PXD064183-1

PXD064183 is an original dataset announced via ProteomeXchange.

Title	Urinary proteome and metabolome uncover tumor microenvironment and cellular metabolism changes of renal clear cell carcinoma
Description	We applied LC-MS to profile the urine proteome and metabolome of 314 ccRCC, 341 healthy control and 49 kidney benign disease enrolled from three cohorts to a great depth. Functionally, we revealed significant changes of extracellular matrix (ECM) organization, complement and coagulation cascades, amino acid metabolism and fatty acid metabolism in ccRCC. Further cell origin annotation of cancer associated proteins revealed the potential role of myofibroblast cell, pericyte cell, vascular associated smooth muscle cell, and endothelial cell during ECM organization. Protein-protein correlation analysis suggested potential cell-cell communications in tumor TME, especially for tumor and myofibroblast cell. Finally, we discovered four potential urinary biomarkers, FGB,CILP, ITIH1, and GUCA2B, and established a model for ccRCC diagnosis with the AUC value of 0.84 in an external cohort. The model also showed discriminatory ability for ccRCC and benign with the AUC value of 0.75. Present study provided urinary molecular changes, which could reflect TME disorder and cellular metabolism reprogramming
HostingRepository	iProX
AnnounceDate	2025-05-22
AnnouncementXML	Submission_2025-05-22_01:16:26.645.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyan Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 01:15:41	ID requested
⏵ 1	2025-05-22 01:16:27	announced

Dataset with its publication pending

submitter keyword: Urine,proteomics,metabolomics, renal clear cell carcinoma

Wei Sun
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College
contact email	sunwei1018@sina.com
lab head
Xiaoyan Liu
contact affiliation	Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College
contact email	xiaoyanliu.good@163.com
dataset submitter

iProX dataset URI