PXD064174-1

PXD064174 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Overexpression of the signaling coordinator GAB2: a missing link in AML progression
Description	TurboID experiments in pre-leukemic bone marrow cells of various genotypes, including wildtype, Dnmt3aR878H/+ and DnmtaR878H/+ x Npm1cA/+. We performed proximity labeling experiments using the TurboID system to label intracellular proteins that are in close proximity to GAB2, NPM1wt, NPM1cA, DNMT3Awt or DNMT3AR882H protein (baits), in primary murine HSPCs. We used a TurboID cDNA fused in frame to the N- or C-terminus of each bait in an MSCV-based retroviral vector, and transduced these vectors into primary murine bone marrow HSPCs from relevant genotypes. TurboID cDNA alone was used as a control for non-specific biotin labeling. Cells were lysed and labeled proteins were captured using streptavidin beads, followed by on-bead digest and mass spectrometry.
HostingRepository	MassIVE
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_21:36:45.530.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Sprung
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine; Gln->pyro-Glu; S-carboxamidomethyl-L-cysteine
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-21 18:31:16	ID requested
⏵ 1	2025-07-10 21:36:45	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Leukemia, timsTOF, Mouse, Bone marrow, TurboID, DatasetType:Proteomics

submitter keyword: Leukemia, timsTOF, Mouse, Bone marrow, TurboID, DatasetType:Proteomics

Contact List

Timothy J. Ley
contact affiliation	Washington University in St. Louis
contact email	timley@wustl.edu
lab head
Robert Sprung
contact affiliation	Washington University School of Medicine
contact email	robert.sprung@wustl.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097966/