PXD064165 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HCR-Proxy analysis of site-specific pre-rRNA proximal proteomes. |
| Description | We developed a novel method termed HCR-Proxy, a proximity labeling strategy that couples Hybridization Chain Reaction (HCR)-mediated signal amplification with in situ free radical-based biotinylation (Proxy-based labelling), allowing proteomic mapping of RNA-associated proteins with subcompartmental spatial resolution. To benchmark the method, we applied HCR-Proxy to specific sequences of nascent pre-ribosomal RNA (pre-rRNA), enabling the dissection of distinct proteomic landscapes across nucleolar subcompartments associated with distinct pre-rRNA maturation and processing steps. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-04 |
| AnnouncementXML | Submission_2026-02-03_16:23:03.510.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Faraz Mardakheh |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-21 12:43:15 | ID requested | |
| ⏵ 1 | 2026-02-03 16:23:04 | announced | |
Publication List
Keyword List
| submitter keyword: LC-MS/MS |
| Label-free quantitative proteomics |
| Spatial proteomics |
| RNA-proximity labelling |
Contact List
| Faraz Mardakheh |
|---|
| contact affiliation | Department of Biochemistry University of Oxford South Parks Road Oxford OX1 3QU The United Kingdom. |
| contact email | faraz.mardakheh@bioch.ox.ac.uk |
| lab head | |
| Faraz Mardakheh |
|---|
| contact affiliation | Department of Biochemistry,
University of Oxford |
| contact email | f.mardakheh@qmul.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064165 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064165
- Label: PRIDE project
- Name: HCR-Proxy analysis of site-specific pre-rRNA proximal proteomes.
