PXD064140-1

PXD064140 is an original dataset announced via ProteomeXchange.

Title	Pumillio-Pumillio-2 is involved in the regulation of human ovarian granulosa tumor cells2 is involved in the regulation of human ovarian granulosa tumor cells
Description	The RNA-binding protein Pumillio-2 (encoded by PUM2) is active during embryonic development and cell differentiation, but possible roles in the dynamic human ovary or in ovarian granulosa cell (GC)-derived tumors, were not known. We studied KGN cells, which are derived from a human GC tumor. We employed siRNA to down-regulate PUM2. A proteomic analysis showed that this treatment reduced not only PUM2 levels but also the abundance of additional 50 proteins, while more than 60 proteins were increased in abundance. GO term analysis revealed that the pathway mainly increased was cell division, a result, which correlated with an increased proliferation rate of siRNA treated KGN cells, as determined by ATP assay and cell counting. Analysis of cell migration (scratch assays) revealed an increased ability to close the gap but unaffected individual cell velocity or migration direction. Taken together, the results indicate a fundamental role of PUM2 in the regulation of ovarian KGN cells. As PUM2 was also detected in primary GC tumor samples, albeit in variable amounts, PUM2 expression may be a novel marker, which may allow to distinguish subsets of GC tumor cells.
HostingRepository	PRIDE
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-02_05:00:33.261.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-21 03:52:27	ID requested
⏵ 1	2026-07-02 05:00:34	announced

10.1093/REPROD/XAAG075;

submitter keyword: human ovary,RNA-binding protein, Granulosa tumor cells, proliferation

Prof. Dr. med. Artur Mayerhofer
contact affiliation	Professorship of Molecular Anatomy Division of Cell Biology/Anatomy III Biomedical Center University of Munich (LMU) Großhaderner Str. 9 82152 Planegg-Martinsried Germany +49 89 2180 75859
contact email	mayerhofer@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD064140

PRIDE project URI

• PRIDE
  1. PXD064140
     1. Label: PRIDE project
     2. Name: Pumillio-Pumillio-2 is involved in the regulation of human ovarian granulosa tumor cells2 is involved in the regulation of human ovarian granulosa tumor cells