PXD064135-1

PXD064135 is an original dataset announced via ProteomeXchange.

Title	Global proteomic analysis reveals key proteins through which miR-146a regulates NO production in the context of the inflammation pathway
Description	Inflammation, while essential for host defense, can become detrimental when dysregulated. MicroRNA-146a (miR-146a) is a well-known anti-inflammatory regulator, primarily targeting the NF-κB pathway. However, its comprehensive mechanisms remain incompletely understood. This study aimed to elucidate the broader impact of miR-146a overexpression on the proteome of lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages using quantitative proteomics. Transfection with a miR-146a mimic resulted in its significant upregulation and the downregulation of known targets, Traf6 and Irak1, at the mRNA level. Global proteomic analysis identified 1232 differentially expressed proteins (DEPs). Pathway enrichment analysis revealed downregulation of proteins associated with IL-6 signaling and NF-κB-related transcription factors (Rel, Nfkb2, Cebpb), alongside decreased expression of pro-inflammatory mediators (Nlrp3, Nos2, Ptgs2). Interestingly, downregulation of Stat2 and interferon-stimulated genes, as well as upregulation of type II interferon signaling components, were also observed. Quantitative RT-PCR and parallel reaction monitoring (PRM) largely validated the proteomic findings for Ptgs2, S100a8, Nos2, Mapkapk2, and Irf3. Functionally, miR-146a overexpression significantly reduced LPS-induced nitric oxide and IL-6 production. These findings demonstrate that miR-146a exerts multifaceted anti-inflammatory effects in macrophages beyond its direct targets in the NF-κB pathway, impacting key inflammatory mediators and potentially modulating interferon signaling, thus providing novel insights into its regulatory mechanisms and therapeutic potential.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:13:13.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Poorichaya Somparn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-21 02:23:28	ID requested
⏵ 1	2026-09-06 16:13:14	announced

10.3390/ijms27146514;

Tangwattanachuleeporn M, Srichaimongkol A, Makjaroen J, Virakul S, Nanthawong S, Sun HS, Dewi NNA, Palaga T, Wongsurawat T, Leelahavanichkul A, Somparn P, Global Proteomic Analysis Reveals Inflammatory Pathway Modulation Associated with miR-146a in LPS-Stimulated Macrophages. Int J Mol Sci, 27(14):(2026) [pubmed]

submitter keyword: Nitric oxide,Inflammation, Proteomics, Lipopolysaccharide, miR-146a

Poorichaya Somparn
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
contact email	poorichaya.s@chula.ac.th
lab head
Poorichaya Somparn
contact affiliation	Chulalongkorn university
contact email	poorichaya.s@chula.ac.th
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD064135

PRIDE project URI

• PRIDE
  1. PXD064135
     1. Label: PRIDE project
     2. Name: Global proteomic analysis reveals key proteins through which miR-146a regulates NO production in the context of the inflammation pathway