PXD064120-1

PXD064120 is an original dataset announced via ProteomeXchange.

Title	Astrogliosis and miscommunication in ALS
Description	Chronic activation of glial cells leads to the dysfunction and degeneration of motor and cortical neurons in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) with an unknown mechanism. To shed light on the molecular pathogenetic processes underlying the exordium and contribution of gliosis to disease onset and progression, we used cells, mice, and patient-derived cells modeling TDP-43, SOD1, and C9orf72-linked and sporadic ALS. We identify an initial phase of the disease marked by increased astrocyte proliferation, followed by a typical activation stage with elevated expression of pro-inflammatory genes. Using mouse genetics, we show that expression of mutant TDP-43 in astrocytes is necessary to cause gliosis and behavioral abnormalities. Mechanistically, we show that c-Myc gain-of-function drives astrocyte proliferation by promoting the release of EVs that nonetheless fail to provide trophic support to surrounding neurons. Our research reveals a novel functional role for c-Myc in astrocytes in the miscommunication from glia to neurons in ALS.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_17:38:27.850.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD064120
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romina Belli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-20 11:17:30	ID requested
⏵ 1	2026-06-28 17:38:28	announced

Fioretti PV, Barbieri A, Migazzi A, Bressan D, Grassano M, Donini L, Roccuzzo M, Torrieri MC, Conci F, Ferracci E, Invernizzi S, Bowden KM, Bacchetti F, Cappelli S, Peroni D, Belli R, Pancher M, Mugoni V, Scarduelli G, Gianesello M, Pasetto L, Canarutto G, Carra S, Soldano A, Bisio A, Robbiati S, Valentini C, Nardella C, Piazza S, D'Agostino VG, Quattrone A, Sleiman S, Whitfield JR, Soucek L, Vignoli B, Viero G, Tiberi L, Zippo A, Demichelis F, Bonetto V, Milanese M, Buratti E, Verde F, Ticozzi N, Calvo A, Ratti A, Shaw PJ, Terenzio M, Chiacchiera F, Pennuto M, Basso M, MYC-driven gliosis impairs neuron-glia communication in amyotrophic lateral sclerosis. Brain, 149(5):1604-1622(2026) [pubmed]

10.6019/PXD064120;

10.1093/brain/awaf360;

submitter keyword: None

Manuela Basso
contact affiliation	Laboratory of neurobiology and pathology, Department of Cellular, Computational and Integrative Biology (DeCIBIO), University of Trento, Italy
contact email	manuela.basso@unitn.it
lab head
Romina Belli
contact affiliation	University of Trento
contact email	romina.belli@unitn.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064120

PRIDE project URI

• PRIDE
  1. PXD064120
     1. Label: PRIDE project
     2. Name: Astrogliosis and miscommunication in ALS