PXD064111 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lipid nanotubes unmask neutrophils for complement attack |
| Description | Neutrophils, critical components of innate immunity, undergo significant morphological changes during phagocytosis. In this study, we demonstrate that neutrophils exposed to shear stress generate lipid nanotubes (NTs) with a unique composition. Compared to the proteome of whole neutrophils, NTs notably lack cytoskeletal elements and the complement-inhibiting transmembrane protein CD46. Consequently, these NTs are recognized by the complement system and selectively opsonized the complement component C3b. Biophysical characterization of NTs confirmed that their integrity relies on lipid-lipid interactions and that they pinch off from neutrophils to form NT-derived vesicles (NTDVs). We detected C3b+ NTDVs in plasma from patients and animal models experiencing diverse inflammatory conditions, including metastatic melanoma, vasculitis, and polytrauma. Further functional experiments indicate that resting neutrophils phagocytose complement-opsonized NTDVs, leading to cell activation, including the production of reactive oxygen species (ROS). In conclusion, our data suggest a significant role for neutrophil-derived NTs in a wide range of inflammatory diseases and reveal a previously unknown mode of cell-cell communication. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:17:23.975.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bente Siebels |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-20 08:10:42 | ID requested | |
| ⏵ 1 | 2026-09-06 16:17:24 | announced | |
Publication List
| 10.1182/blood.2025029974; |
| Liu X, Wang Y, Bauer AT, Marki A, Krebs CF, H, ä, ffner K, Siebels B, Schl, ü, ter H, Hildebrand F, Halbgebauer R, Huber-Lang M, Ley K, Schneider MF, Schneider SW, Gorzelanny C, Lipid nanotubes unmask neutrophils for complement attack. Blood, 147(12):1338-1350(2026) [pubmed] |
Keyword List
| submitter keyword: complement system, shear stress, extracellular vesicles,Neutrophils, polytrauma, vasculitis |
Contact List
| Christian Gorzelanny |
|---|
| contact affiliation | Department of Dermatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany |
| contact email | c.gorzelanny@uke.de |
| lab head | |
| Bente Siebels |
|---|
| contact affiliation | Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf |
| contact email | b.siebels@uke.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064111
- Label: PRIDE project
- Name: Lipid nanotubes unmask neutrophils for complement attack
