PXD064097 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Streamlined isolation of the n-terminome |
| Description | SIMPATICO is a streamlined N-termini proteome workflow which leverages NHS ester chemistry. At the protein level, N-terminal amines (naturally occurring protein N-termini, lysines, or protease-generated N-termini) are blocked directly in the cell lysate, and tryptic digestion is performed straight after, without the need for any buffer exchange. The internal tryptic peptides are tagged with a phosphonate moiety and subsequently depleted via metal-based coordination, keeping exclusively N-blocked terminal peptides for analysis by LC-MS/MS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-04 |
| AnnouncementXML | Submission_2026-09-04_01:47:18.257.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Piero Giansanti |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-20 03:22:06 | ID requested | |
| ⏵ 1 | 2026-09-04 01:47:19 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: TMT, LFQ, proteolysis,n-terminome, degradomics |
Contact List
| Piero Giansanti |
|---|
| contact affiliation | Bavarian Center for Biomolecular Mass Spectrometry at Klinikum rechts der Isar - Technical University of Munich |
| contact email | piero.giansanti@tum.de |
| lab head | |
| Piero Giansanti |
|---|
| contact affiliation | Technical University of Munich |
| contact email | piero.giansanti@tum.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD064097 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064097
- Label: PRIDE project
- Name: Streamlined isolation of the n-terminome
