PXD064055-1

PXD064055 is an original dataset announced via ProteomeXchange.

Title	Systematic Discovery and Design of Synthetic Immune Receptors in Plants
Description	Plants deploy a diverse array of pattern recognition receptors (PRRs) that perceive microbe-associated molecular patterns (MAMPs) to activate immune responses. Leucine-rich repeat receptor-like kinase subgroup XII (LRR-RLK-XII) represents one of the largest PRR families due to lineage-specific diversification. Bioinformatics, synthetic biology, and biochemical approaches were integrated to characterize PRRs from 285 plant species. We identified a receptor, named “SCORE”, that perceives cold-shock protein (CSP) peptides. SCORE orthologs from multiple angiosperm lineages exhibit remarkable CSP recognition polymorphisms, indicating recurrent selection for pathogen recognition through substitutions at key amino acid residues. Through functional phylogenomics and protein structure predictions, we generated synthetic SCORE variants capable of detecting multiple phytopathogen CSP peptides, thus revealing the diverse PRR recognition landscape in plants. Our strategy hold promises for engineering plant immune receptors, particularly for perennial crops.
HostingRepository	PRIDE
AnnounceDate	2025-06-12
AnnouncementXML	Submission_2025-06-11_16:53:53.717.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064055
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Takehiro Suzuki
SpeciesList	scientific name: Agrobacterium fabrum str. C58; NCBI TaxID: 176299;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-19 03:41:28	ID requested
⏵ 1	2025-06-11 16:53:54	announced

10.6019/PXD064055;

submitter keyword: Plants,Synthetic Immune Receptors, Agrobacterium

Naoshi Dohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064055
     1. Label: PRIDE project
     2. Name: Systematic Discovery and Design of Synthetic Immune Receptors in Plants