PXD063994 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optimization of a method to quantify S-acylation |
| Description | HeLa cells were lysed and free cysteines were alkylated with an alkyne-tagged light probe (IAA-BnL or IAA-PrL). S-acylation was hydrolysed with hydroxylamine and newly revealed Cys allowed to react with the heavy probe (IAA-BnL or IAA-PrL). Proteins were clicked with azido-biotin, Adadps-biotin, AazoB or ARB, enriched on neutravidin beads, digested with trypsin and desalted. Different optimisations of the protocol were tested: -8 sets of probes / capture reagent -LC-MS/MS optimisation (gradient, FAIMS, etc) Finally, S-acylation was quantified for a biological replicate of HeLa cells. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-29_05:27:19.452.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | EMMANUELLE THINON |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | isotope tagged reagent derivatized residue |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-15 14:38:46 | ID requested | |
| ⏵ 1 | 2026-06-29 05:27:19 | announced | |
Publication List
Keyword List
| submitter keyword: HeLa,S-acylation |
Contact List
| Emmanuelle Thinon |
|---|
| contact affiliation | Univ. Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, IECB, F-33600 Pessac, France |
| contact email | e.thinon@iecb.u-bordeaux.fr |
| lab head | |
| EMMANUELLE THINON |
|---|
| contact affiliation | CNRS |
| contact email | e.thinon@iecb.u-bordeaux.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD063994 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063994
- Label: PRIDE project
- Name: Optimization of a method to quantify S-acylation
