PXD063965-1

PXD063965 is an original dataset announced via ProteomeXchange.

Title	Rabies virus P protein binds to TBK1 and interferes with the formation of innate immunity-related liquid condensates
Description	Viruses must overcome the interferon-mediated antiviral response to replicate and propagate into their host. Rabies virus (RABV) phosphoprotein P is known to inhibit interferon induction. Here, using a global mass spectrometry approach, we show that RABV P binds to TBK1, a kinase located at the crossroads of many interferon induction pathways, resulting in innate immunity inhibition. Mutations of TBK1 phosphorylation sites abolish P binding. Importantly, we demonstrate that upon RABV infection or detection of dsRNA by innate immunity sensors, TBK1 and its adaptor proteins NAP1 and SINTBAD form dynamic cytoplasmic condensates that have liquid properties. These condensates can form larger aggregates having ring-like structures in which NAP1 and TBK1 exhibit locally restricted movement. P binding to TBK1 interferes with the formation of these structures. This work demonstrates that proteins of the signaling pathway leading to interferon induction transiently form liquid organelles that can be targeted by viruses.
HostingRepository	PRIDE
AnnounceDate	2025-09-11
AnnouncementXML	Submission_2025-09-11_07:28:02.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	half cystine; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-15 03:06:41	ID requested
⏵ 1	2025-09-11 07:28:02	announced

10.1016/J.CELREP.2022.111949;

submitter keyword: viral,TBK1, virus, interferon, factories, liquid condensates, Rabies

Cécile, LAGAUDRIERE-GESBERT
contact affiliation	Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, France
contact email	cecile.lagaudriere@i2bc.paris-saclay.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063965
     1. Label: PRIDE project
     2. Name: Rabies virus P protein binds to TBK1 and interferes with the formation of innate immunity-related liquid condensates