PXD063962-1

PXD063962 is an original dataset announced via ProteomeXchange.

Title	Dysregulation of RAS degradation limits the efficacy of RAS inhibitors via reprogramming amino acid sensing machinery
Description	To investigate the molecular consequences of LZTR1 loss and its impact on KRAS-regulated signaling, we performed deep proteomic and phosphoproteomic profiling using mass spectrometry. Human tumor cells with CRISPR/Cas9-mediated LZTR1 knockout and their isogenic controls were subjected to tandem mass tag (TMT)-based quantitative proteomic and phosphoproteomic analysis. In parallel, we isolated tumor cells from genetically engineered mouse models harboring KRAS mutations and analyzed their global proteome using label-free quantitative proteomics. These complementary approaches enabled us to systematically characterize changes in protein abundance and phosphorylation status associated with LZTR1 deficiency and to identify pathways potentially involved in resistance to KRAS-targeted therapies.
HostingRepository	PRIDE
AnnounceDate	2025-10-09
AnnouncementXML	Submission_2025-10-08_23:36:15.687.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD063962
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	raj sewduth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-15 02:23:47	ID requested
⏵ 1	2025-10-08 23:36:16	announced

10.6019/PXD063962;

submitter keyword: MTOR,LUAD, KRAS

Anna Sablina
contact affiliation	VIB-KU Leuven Center for Cancer Biology
contact email	anna.sablina@kuleuven.be
lab head
raj sewduth
contact affiliation	VIB KU Leuven
contact email	raj.sewduth@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063962
     1. Label: PRIDE project
     2. Name: Dysregulation of RAS degradation limits the efficacy of RAS inhibitors via reprogramming amino acid sensing machinery