PXD063956-1

PXD063956 is an original dataset announced via ProteomeXchange.

Title	De novo designed protein guiding targeted protein degradation
Description	Targeted protein degradation is a powerful tool for biological research, cell therapy, and synthetic biology. However, conventional methods often depend on pre-fused degrons or chemical degraders, limiting their wider applications. In this study, a guided protein labeling and degradation system (GPlad) was developed in Escherichia coli, using de novo designed guide proteins and arginine kinase (McsB) for precise degradation of various proteins, including fluorescent proteins, metabolic enzymes, and human proteins. GPlad was expanded into versatile tools such as antiGPlad, OptoGPlad, and GPTAC, enabling reversible inhibition, optogenetic regulation, and biological chimerization. The combination of GPlad and antiGPlad allows for programmable circuit construction, such as ON/OFF switches, signal amplifiers, and oscillators. OptoGPlad-mediated degradation of MutH accelerated E. coli evolution under protocatechuic acid stress, reducing the required generations from 220 to 100. Additionally, GPTAC-mediated degradation of AroE enhanced the titer of 3-dehydroshikimic acid to 92.6 g/L, a 23.8% improvement over the conventional CRISPR interference method. GPlad provides a tunable, plug-and-play strategy for straightforward protein degradation without the need for pre-fusion, with substantial implications for synthetic biology and metabolic engineering
HostingRepository	PRIDE
AnnounceDate	2025-07-05
AnnouncementXML	Submission_2025-07-04_22:34:38.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhendong Li
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-15 00:55:49	ID requested
⏵ 1	2025-07-04 22:34:38	announced

Dataset with its publication pending

submitter keyword: Protein degradation

Protein design

Zhendong Li
contact affiliation	Jiangnan university
contact email	7200201049@stu.jiangnan.edu.cn
lab head
zhendong Li
contact affiliation	Jiangnan university
contact email	7200201049@stu.jiangnan.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063956
     1. Label: PRIDE project
     2. Name: De novo designed protein guiding targeted protein degradation