PXD063944-1

PXD063944 is an original dataset announced via ProteomeXchange.

Title	Synergistic Regulation by ClpX and PstB Modulates Susceptibility to Quaternary Ammonium Compounds in Streptococcus gordonii via Competence-Driven Metabolic Adaptation
Description	Overuse of cationic biocides including quaternary ammonium compounds (QACs) poses a growing threat by selecting for antimicrobial resistance. Here, we use Streptococcus gordonii, a commensal opportunistic pathogen, as a model to elucidate the mechanisms of QAC lethality and resistance. We show that QACs trigger V-type ATPase–driven metabolic dysfunction, leading to oxidative phosphorylation (OXPHOS) dominance and excessive reactive oxygen species (ROS) accumulation. Resistance arises through synergistic regulation by ClpX and PstB, which repress the competence pathway regulator ComDE and reprogram metabolism toward aerobic glycolysis, thereby limiting ROS production. Mechanistically, ClpX modulates ComDE signaling through protein aggregation and HtrA-mediated degradation of competence-stimulating peptide, while PstB governs ComDE via phosphorylation of the mannose-specific phosphotransferase system. Activation of ComDE restores ROS-mediated QAC lethality, identifying this pathway as a potential therapeutic target. These findings reveal that metabolic adaptation driven by competence regulon governs biocide susceptibility and adaptation, offering new strategies to counteract QAC resistance and associated cross-resistance in commensal bacteria.
HostingRepository	iProX
AnnounceDate	2025-05-14
AnnouncementXML	Submission_2025-06-29_22:52:13.979.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hao Li
SpeciesList	scientific name: Streptococcus gordonii str. Challis substr. CH1; NCBI TaxID: 467705;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-14 20:49:13	ID requested
⏵ 1	2025-06-29 22:52:14	announced

Dataset with its publication pending

submitter keyword: ROS

Quaternary Ammonium Compounds

Cationic biocides；competence

Biao Ren
contact affiliation	Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University
contact email	renbiao@scu.edu.cn
lab head
Hao Li
contact affiliation	Department of Endodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University
contact email	lihao931006@gmail.com
dataset submitter

iProX dataset URI