PXD063909 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Biogenesis and migration of the sperm cytoplasmic droplet requires ARRDC5 and TEX38 |
| Description | The maturation of spermatid to fertilization competent spermatozoa is a sophisticated process required for sexual reproduction, and biogenesis of the cytoplasmic droplet (CD) is a key event. Here we demonstrate that two proteins with testis-specific expression, ARRDC5 and TEX38, physically interact, are required for normal sperm maturation, and play a critical role in CD formation and function. In the CDs of the Arrdc5-/- or Tex38-/- mice, a key feature of saccular element biogenesis fails, the proteome is abnormal, and migration from the neck down the flagellum does not occur. Furthermore, comparative proteomic profiling between sperm CDs and spermatids from Arrdc5-/-, Tex38-/-, and wild-type mice suggests that ARRDC5 and TEX38 facilitate cargo loading during CD biogenesis and deposition of proteins such as TOMM40 from the CD to the sperm mitochondrial sheath during epididymal maturation. This protein delivery mechanism is likely a key event in enabling sperm motility and therefore fertilization capacity. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-02 |
| AnnouncementXML | Submission_2026-03-01_16:59:44.527.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mingyao Yang |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-13 19:30:42 | ID requested | |
| ⏵ 1 | 2026-03-01 16:59:45 | announced | |
Publication List
| 10.1016/j.celrep.2025.116731; |
| Yang M, Oatley MJ, Miao D, Maddison LA, Oatley JM, Biogenesis and migration of the sperm cytoplasmic droplet require ARRDC5 and TEX38. Cell Rep, 45(1):116731(2026) [pubmed] |
Keyword List
| submitter keyword: cytoplasmic droplet, LC-MS, Arrdc5-/- or Tex38-/- mice, spermatid, spermatozoa |
Contact List
| Jon Oatley |
|---|
| contact affiliation | Washington State University, College of Veterinary Medicine, School of Molecular Biosciences, |
| contact email | joatley@wsu.edu |
| lab head | |
| Mingyao Yang |
|---|
| contact affiliation | Washington State University |
| contact email | mingyaoyang0325@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD063909 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063909
- Label: PRIDE project
- Name: Biogenesis and migration of the sperm cytoplasmic droplet requires ARRDC5 and TEX38
