PXD063902-1

PXD063902 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Beta-cell Galphas signaling is critical for physiological and pharmacological enhancement of insulin secretion
Description	Islet lysates were adjusted to 5 percent SDS in TEAB, pH 8.5, reduced and alkylated and digested with modified trypsin (Worthington) using an S-trap mini device (Protifi). Peptides were labeled with TMT16 reagents (lot ZH350094). Phosphopeptide enrichment used GL Sciences p10 TiO tips. LC-MS/MS used a MClass UPLC system (Waters) and Exploris 480 (ThermoFisher). Analytical separation used HSS T3 C18 75 micron by 250 mm column (Waters) with a 90 min gradient of 5 to 30 percent MeCN at a flow rate of 400 nl per min. Data collection on TMT-labeled samples was performed in data-dependent acquisition (DDA) mode with 2 FAIMS compensation voltages. TMT data was analyzed using Fragpipe 19.
HostingRepository	MassIVE
AnnounceDate	2025-06-06
AnnouncementXML	Submission_2025-06-06_05:33:40.849.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl; Phospho; TMTpro
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-13 17:49:18	ID requested
⏵ 1	2025-06-06 05:33:41	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: IBMX, phosphorylation, islet, DatasetType:Proteomics

submitter keyword: IBMX, phosphorylation, islet, DatasetType:Proteomics

Contact List

Jonathan Campbell
contact affiliation	Duke University
contact email	jonathan.campbell@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097882/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097882/