PXD063852 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multisite phosphorylation of intrinsically disordered region of DVL |
| Description | When WNT binds to its receptor Frizzled (FZD) to activate the signalling, Dishevelled (DVL), the central component of Wnt signalling, is phosphorylated by redundant Casein kinase 1 (CK1) isoforms ε or δ, to transduce the signal downstream. Although the basic principles of Wnt signal transduction are known, the mechanistic aspects of DVL phosphorylation remain elusive. Here we identify Wnt-dependent multisite phosphorylation of a large and conserved serine/threonine (S/T) cluster within intrinsically disordered region (IDR), located between the PDZ and DEP domains. The detailed analysis of the phosphorylation and its effect on the flanking domains was performed with the construct containing both domains PDZ and DEP flanking the IDR (hDVL3 PDZ-DEP, aa 243-496). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-07 |
| AnnouncementXML | Submission_2026-04-07_05:10:21.821.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Katerina Hanakova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-12 04:05:03 | ID requested | |
| ⏵ 1 | 2026-04-07 05:10:22 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Dishevelled, phosphorylation |
Contact List
| Zbynek Zdrahal |
|---|
| contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
| contact email | zdrahal@sci.muni.cz |
| lab head | |
| Katerina Hanakova |
|---|
| contact affiliation | CEITEC MU |
| contact email | katerina.hanak@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD063852
- Label: PRIDE project
- Name: Multisite phosphorylation of intrinsically disordered region of DVL
