PXD063824-1

PXD063824 is an original dataset announced via ProteomeXchange.

Title	Polydopamine nanoparticles as a potential non-pharmaceutical antioxidant tool against mitochondrial disorders
Description	Mitochondrial disorders are a subset of hereditary diseases characterized by defects in mitochondrial functionality due to mutations in genes in nuclear or mitochondrial DNA. The molecular hallmarks of mitochondrial disorders include a high production of reactive oxygen species (ROS), morphological and functional mitochondrial aberrations, and metabolic impairments. From a systemic perspective, these molecular and cellular impairments can lead to severe disorders affecting the central nervous system, besides to muscles, heart, and gastrointestinal problems. The treatment of mitochondrial diseases with antioxidant moieties like idebenone and resveratrol has been proposed and tested as a potential therapy for these disorders. Polydopamine nanoparticles (PDNPs) are nanostructures derived from the oxidative self-polymerization of dopamine and characterized by high biocompatibility and biodegradability, the ability to be easily functionalized with active molecules, high antioxidant properties, and the ability to act as photothermal conversion agents when irradiated with near infrared (NIR) light. In this work we tested the potential of PDNPs as a non-pharmaceutical treatment for two mitochondrial diseases, namely mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) and progressive external ophthalmoplegia (PEO). We tested PDNPs on fibroblasts derived from healthy subjects and fibroblasts derived from MELAS and PEO patients. The cells were first characterized from a molecular point of view through proteomic analysis. Then, the biocompatibility, internalization rate, intracellular localization, and protective antioxidant properties of PDNPs were assessed. Lastly, the antioxidant properties of PDNPs were also confirmed on an in vivo model based on zebrafish embryos. Our data demonstrates the ability of PDNPs to protect both healthy and patient-derived cells from ROS-induced damage, thus preventing the increment of oxidative stress, ROS-induced apoptosis, and mitochondrial dysfunctions. Eventually, PDNPs showed the capacity to protect zebrafish embryos from pro-oxidative stimuli. Altogether, this work demonstrates the potential of polydopamine nanostructures as an effective countermeasure for some of the molecular hallmarks associated with mitochondrial disorders, paving the way for the future exploitation in clinical applications.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_03:18:36.337.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-09 16:23:52	ID requested
⏵ 1	2026-07-16 03:18:36	announced

10.1021/ACSANM.5C03169;

submitter keyword: Poydopamine nanoparticles

mitochondrial diseases

oxidative stress

mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes

progressive external ophthalmoplegia

Andrea Petretto
contact affiliation	IRCCS Istituto G Gaslini
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063824
     1. Label: PRIDE project
     2. Name: Polydopamine nanoparticles as a potential non-pharmaceutical antioxidant tool against mitochondrial disorders