PXD063788-1

PXD063788 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative Proteomic Analysis of Extracellular Vesicles Derived from γ-Irradiated Normal Human Breast Fibroblasts
Description	Small extracellular vesicles (EVs) were isolated from a model of normal breast fibroblasts— immortalized cells derived from healthy reduction mammoplasty tissue—treated with γ-radiation to study the effects of radiation therapy on intercellular communication within the breast stroma. EVs were isolated using the gold-standard differential speed centrifugation protocol. Proteins were subsequently extracted, and a quantitative examination of the alterations in the EV proteome was pursued. Both label-free and isobaric label-based methodologies were conducted to comprehensively characterize proteomic changes.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_16:19:01.931.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristie Lindsey Rose
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 21:58:57	ID requested
⏵ 1	2026-03-01 16:19:02	announced

Publication List

10.1002/pmic.70103;
Berumen S, á, nchez G, Patel P, Rose KL, Rafat M, Proteomics in Practice: A Case Study Highlighting Tandem Mass Tag-Based MS for Quantitative Profiling of Extracellular Vesicles and Application to Irradiated Fibroblasts. Proteomics, 26(2-3):163-177(2026) [pubmed]

10.1002/pmic.70103;

Berumen S, á, nchez G, Patel P, Rose KL, Rafat M, Proteomics in Practice: A Case Study Highlighting Tandem Mass Tag-Based MS for Quantitative Profiling of Extracellular Vesicles and Application to Irradiated Fibroblasts. Proteomics, 26(2-3):163-177(2026) [pubmed]

Keyword List

submitter keyword: extracellular vesicles, comparative proteomics, TMT labeling, ionizing radiation, label-free quantification

submitter keyword: extracellular vesicles, comparative proteomics, TMT labeling, ionizing radiation, label-free quantification

Contact List

Kristie Lindsey Rose
contact affiliation	Vanderbilt University Proteomics Core
contact email	kristie.rose@vanderbilt.edu
lab head
Kristie Lindsey Rose
contact affiliation	Vanderbilt University
contact email	kristie.rose@vanderbilt.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD063788
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD063788

PRIDE project URI

Repository Record List

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